MilliporeSigma
All Photos(2)

Documents

M9901

Sigma-Aldrich

Mutanolysin from Streptomyces globisporus ATCC 21553

lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified

Sign Into View Organizational & Contract Pricing

Synonym(s):
Mutanolysin
CAS Number:
MDL number:
NACRES:
NA.54

Quality Level

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SRE0007M4782SAE0092
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

23 kDa

mol wt

23 kDa

mol wt

23 kDa

mol wt

23 kDa, ~18.5 kDa

Quality Level

300

Quality Level

400

Quality Level

200

Quality Level

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

Application

Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Legal Information

Fiberfrax is a registered trademark of Unifrax Corp.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Pierre Godessart et al.
Nature microbiology, 6(1), 27-33 (2020-11-04)
Gram-negative bacteria are surrounded by a cell envelope that comprises an outer membrane (OM) and an inner membrane that, together, delimit the periplasmic space, which contains the peptidoglycan (PG) sacculus. Covalent anchoring of the OM to the PG is crucial
Amelia T Soderholm et al.
Frontiers in cellular and infection microbiology, 8, 160-160 (2018-06-06)
Streptococcus pyogenes (Group A Streptococcus; GAS) commonly causes pharyngitis in children and adults, with severe invasive disease and immune sequelae being an infrequent consequence. The ability of GAS to invade the host and establish infection likely involves subversion of host
Helena Žemličková et al.
Journal of medical microbiology, 67(7), 1003-1011 (2018-06-02)
Purpose. The aim of this study was to characterize serogroup 19 isolates resistant to macrolides and/or penicillin found among pneumococci recovered from cases of invasive and respiratory tract disease in the Czech Republic in 2014.Methods. Pneumococcal isolates of serotypes 19A
Mutanolysin for Improved Lysis and Rapid Protoplast Formation in Dairy Streptococci
Kondo, J. and L. McKay
Journal of Dairy Science, 65, 1428-1431 (1982)
G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls

Protocols

This procedure may be used for Mutanolysin products.

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service