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MRN70

Sigma-Aldrich

GenElute mRNA Miniprep Kit

sufficient for 70 purifications

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Synonym(s):
GenElute mRNA Kit, Gen Elute

usage

sufficient for 70 purifications

Quality Level

technique(s)

RNA purification: suitable

storage temp.

15-25°C

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This Item
DMN10MRN10RTN70
usage

sufficient for 70 purifications

usage

sufficient for 10 purifications

usage

sufficient for 10 purifications

usage

sufficient for 70 purifications

MRN10

DMN70

MRN70

RTN10, RTN350, RTN9602, RTN9604

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

Procedures such as cDNA synthesis, expression profiling and others require separation of mRNA from the vastly more abundant rRNA and tRNA. The GenElute mRNA kits provide convenient procedures for isolating polyadenylated mRNA from previously prepared total RNA or directly from mammalian cells and tissues. For direct mRNA preparation, cells or tissues are disrupted with SDS/proteinase K digestion to release RNA and eliminate RNases. Both kit types use oligo dT30 covalently linked to 1 μm polystyrene beads to capture polyadenylated mRNA by hybridization. The polystyrene beads remain suspended during hybridization, eliminating the need for mixing or rocking, as is common for cellulose or magnetic particles. Polystyrene was also chosen because oligo(dT) polystyrene beads yield cleaner mRNA with fewer stringent washing steps than does the more commonly used oligo(dT) cellulose (2 or 3 wash steps versus 10 or more). With the GenElute kits, mRNA-bead complexes are washed on a microcentrifuge spin filter, and eluted into 10 mM Tris-HCL, pH 7.5. mRNA prepared with either kit is suitable for a variety of downstream applications such as Northern blotting, expression array or chip hybridizations and cDNA synthesis and library construction.

Application

GenElute mRNA Miniprep Kit has been used to purify RNA from total RNA and to isolate RNA.
The purified mRNA is ready for Northern analysis, reverse transcription and PCR, labeling for arrays, and other common applications.

Features and Benefits

  • Quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein
  • Performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted
  • One of the most effective methods for isolating total RNA. Purifications can be completed in only one hour starting with fresh tissue or cells

Other Notes

For additional information, please see www.sigma-aldrich.com/mrna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Transcriptome-wide mapping of N(6)-methyladenosine by m(6)A-seq based on immunocapturing and massively parallel sequencing.
Dominissini D
Nature Protocols, 8(1), 176-189 (2013)
K Oishi et al.
Journal of thrombosis and haemostasis : JTH, 4(7), 1566-1574 (2006-07-15)
Diabetes is associated with an excess risk of cardiac events, and one risk factor for infarction is an elevated level of plasminogen activator inhibitor-1 (PAI-1). To evaluate whether the glucocorticoid hormones are involved in the diabetes-induced PAI-1 production, we examined
Enjie Li et al.
eLife, 11 (2022-05-04)
Methyltransferase-like 3 (METTL3) and N6-methyladenosine (m6A) are involved in many types of biological and pathological processes, including DNA repair. However, the function and mechanism of METTL3 in DNA repair and chemotherapeutic response remain largely unknown. In present study, we identified
Marimuthu Kumaravel et al.
Scientific reports, 10(1), 4501-4501 (2020-03-13)
Somatic embryos are comparable to their zygotic counterparts for morphological traits but are derived from somatic cells through various metabolic regulations, collectively referred as somatic embryogenesis (SE). It has been well exploited for germplasm conservation, genetic engineering, mutation breeding, for
Stephen DiGiuseppe et al.
Virology, 458-459, 93-105 (2014-06-15)
The Human papillomavirus (HPV) capsid is composed of the major and minor capsid proteins, L1 and L2, respectively. Infectious entry requires a complex series of conformational changes in both proteins that lead to uptake and allow uncoating to occur. During

Articles

Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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