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N0513

Sigma-Aldrich

Nerve Growth Factor-7S from murine submaxillary gland

NGF-7S, lyophilized powder, suitable for cell culture

Synonym(s):
NGF-7S
CAS Number:
EC Number:
MDL number:
NACRES:
NA.77

biological source

mouse

Quality Level

form

lyophilized powder

potency

2-250 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

protein 130 kDa

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

≤10

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Ngf(18049)

General description

Nerve growth factor (NGF) is a dimer of 118-residue polypeptide chains linked non-covalently. Both these polypeptides contain three intramolecular disulphide bridges. It forms the prototype of the neurotrophin growth factor family. In male mouse submandibular salivary gland, NGF is present in high amounts, and is present as a high molecular weight complex called nerve growth factor-7S (NGF-7S). NGF-7S is an α2β2γ2 complex, where β2-NGF is the active neurotrophin, and is linked to the dimers of the glandular kallikrein serine proteinases members, α-NGF and γ-NGF.[1]

Application

Nerve Growth Factor-7S (NGF-7S) from murine submaxillary gland has been used for the treatment of following cells to induce neuronal differentiation-
  • PC12[2][3]
  • Dorsal root ganglia (DRG) obtained from chick embryos of either sex (Gallus gallus)[4]

Biochem/physiol Actions

Nerve growth factor (NGF) is a neurotrophin, which facilitates the differentiation and survival of specific subtypes of neurons of central nervous system and peripheral nervous system. However, nerve growth factor-7S (NGF-7S) lacks any neurotrophic activity as most part of β-NGF subunit is buried within the NGF-7S complex.[1]

Physical form

Lyophilized from 0.2 μm filtered solution in 25 mM sodium phosphate, pH 7.0.

Preparation Note

Nerve growth factor-7S (NGF-7S) is isolated from male mouse submaxillary glands using a modification of the method of Varon, et al.[5]

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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