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N3755

Sigma-Aldrich

Nuclease micrococcal from Staphylococcus aureus

100-300 units/mg protein

Synonym(s):
Micrococcal nuclease, Endonuclease micrococcal, MNase, Micrococcal endonuclease
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

Staphylococcus aureus

Quality Level

form

lyophilized powder

specific activity

100-300 units/mg protein

composition

Protein, ≥40% E1%/280 (Balance primarily sodium citrate)

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

Staphylococcus aureus subsp. aureus Mu50 ... nuc(1120790)

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Application

Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases.

Packaging

50, 200, 500 units in serum bottle

Biochem/physiol Actions

Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C.
Hydrolyzes 5′-phosphodiester bonds of DNA.

Unit Definition

One unit will produce 1.0 μmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37 °C, based on EM/260 = 10,000 for the mixed nucleotides.

Other Notes

Note: One μmolar unit = approx. 85 A260 units, where an A260 unit is equivalent to a ΔA260 of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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