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N6655

Sigma-Aldrich

Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

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Synonym(s):
Anti-NGF-2.5S
EC Number:
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

Ouchterlony double diffusion: suitable
western blot: 1:2,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

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This Item
N3279SAB1306570N8773
species reactivity

mouse, human

species reactivity

human, rat, mouse

species reactivity

human

species reactivity

rat

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

goat

clone

polyclonal

clone

25623, monoclonal

clone

polyclonal

clone

polyclonal

antibody form

fractionated antiserum

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

UniProt accession no.

P01138

UniProt accession no.

P01138

UniProt accession no.

P01138

UniProt accession no.

P25427

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General description

Nerve growth factor (NGF) belongs to the family of neurotrophic factors. It is located at 1p13.2 on the human chromosome. There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.

Specificity

May be used in monitoring for NGF (2.5S, β and 7S).

Immunogen

NGF-2.5S from male mouse submaxillary glands.

Application

Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats. The antibody was used for immunofluorescence of mouse astrocytes and for neutralization assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).

Physical form

Solution in phosphate buffered saline.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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New protein fold revealed by a 2.3-
McDonald N Q, et al.
Nature, 354(6352), 411-411 (1991)
Liang-Wei Chen et al.
The Journal of comparative neurology, 497(6), 898-909 (2006-06-28)
To address the hypothesis that reactive astrocytes in the basal ganglia of an animal model of Parkinson's disease serve neurotrophic roles, we studied the expression pattern of neurotrophic factors in the basal ganglia of C57/Bl mice that had been treated
7S nerve growth factor has different biological activity from 2.5 S nerve growth factor in vitro
Shao N, et al.
Brain Research, 609(1-2), 338-340 (1993)
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Frontiers in neuroscience, 13, 58-58 (2019-02-23)
Previous studies demonstrated exercise-induced modulation of neurotrophins, such as Nerve Growth Factor (NGF) and Brain-Derived Neurotrophic Factor (BDNF). Yet, no study that we are aware of has examined their change as a function of different training paradigms. In addition, the
Obaida R Rana et al.
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Recently, we provided a technique of chronic high-frequency electric stimulation (HFES) of the right inferior ganglionated plexus for ventricular rate control during atrial fibrillation in dogs and humans. In these experiments, we observed a decrease of the intrinsic ventricular rate

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