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N6655

Sigma-Aldrich

Anti-Nerve Growth Factor 2.5S antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

Synonym(s):
Anti-NGF-2.5S
EC Number:
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

Ouchterlony double diffusion: suitable
western blot: 1:2,000

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NGF(4803)
mouse ... Ngf(18049)

General description

Nerve growth factor (NGF) belongs to the family of neurotrophic factors.[1] It is located at 1p13.2 on the human chromosome.[2] There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.[3]

Specificity

May be used in monitoring for NGF (2.5S, β and 7S).

Immunogen

NGF-2.5S from male mouse submaxillary glands.

Application

Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats.[4] The antibody was used for immunofluorescence of mouse astrocytes[5] and for neutralization assays.[6][7]
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).[2]

Physical form

Solution in phosphate buffered saline.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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