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N7285

Sigma-Aldrich

Ninhydrin Reagent

2% Solution

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Synonym(s):
1,2,3-Indantrione monohydrate, 2,2-dihydroxyindene-1,3-dione, Ninhydrine
EC Number:
NACRES:
NA.56

Quality Level

form

liquid

storage temp.

2-8°C

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This Item
1.06762151173N4876
vibrant-m

N7285

Ninhydrin Reagent

vibrant-m

1.06762

Ninhydrin

vibrant-m

151173

Ninhydrin

vibrant-m

N4876

Ninhydrin

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

-

storage temp.

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Application

Ninhydrin reagent has been used to confirm the presence of the amino acid moieties in various reactions.
Ninhydrin reagents can be used in thin layer chromatography (TLC). Ninhydrin reagents have been used to develop and validate a simple, selective, and precise densitometric method for analysis of α-aminocephalosporins, both in bulk drugs and in formulations. Ninhydrin reagents have also been used to observe differences in the reference ranges of several plasma and urine amino acids between Singaporean and Caucasian populations.

Biochem/physiol Actions

Ninhydrin is a tricyclic 1,2,3-trione, which functions as an amino acid reagent. It is a vital organic building block, which exposes latent fingerprints on porous surfaces like paper, cardboard and raw wood. Ninhydrin is used to detect primary and secondary amines. It gives a strong blue color with primary amines and a less intense brown-red color with secondary amine.

Other Notes

Ninhydrin and hydrindantin in DMSO and lithium acetate buffer, pH 5.2

Caution

Store under nitrogen in the refrigerator after use

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Annals of the Academy of Medicine, Singapore, 35(7), 468-475 (2006-08-12)
The analysis of amino acids in plasma and urine was introduced in Singapore when a laboratory for the investigation of inherited metabolic disorders was established by the Ministry of Health. Reference ranges are required for interpreting test results and making
Gamal A Saleh et al.
Journal of chromatographic science, 48(1), 68-75 (2010-01-09)
A simple, selective, and precise densitometric method for analysis of four alpha-aminocephalosporins, namely cefaclor monohydrate, cefadroxil monohydrate, cefalexin anhydrous, and cefradine anhydrous, both in bulk drugs and in formulations was developed and validated. The method employed thin-layer chromatography (TLC) aluminium

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