P0059

Pro-carboxypeptidase B human

≥2.0 units/mg protein

• Synonym(s): Thrombin Activatable Fibrinolysis Inhibitor (TAFI)
• MDL number: MFCD02689174
• NACRES: NA.77

Properties

• Quality Level: 200
• specific activity: ≥2.0 units/mg protein
• mol wt: 60 kDa
• UniProt accession no.: P15086
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CPB1(1360)

Description

Biochem/physiol Actions

TAFI is supplied in its 60 kDa zymogen form. The N-terminal of TAFI contains four N-linked glycans and a plasminogen recognition sequence. Hydrolysis of the activation peptide by thrombin releases a 36-kDa active proteolytic domain homologous to tissue carboxypeptidase B. Complexation with thrombomodulin increases thrombin activation of TAFI 1250 fold. TAFI exerts it′s inhibition of fibrinolytic activity by binding to plasminogen and by cleaving C-terminal lysine residues of fibrin resulting in the reduction of plasmin and tPA binding sites on fibrin. Experiments in TAFI-deficient mice point to a regulatory role on complement C5a.

Unit Definition

One unit will hydrolyze 1 micromole of Hippuryl-L-Arginine per minute at pH 7.4 at 25 °C.

Physical form

Solution containing 20 mM Hepes and 150 mM Sodium chloride. pH 7.4

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves