Documents
P9620
Puromycin dihydrochloride
Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture
Recommended Products
biological source
Streptomyces alboniger
Quality Level
type
for cell culture
Assay
>98% (HPLC)
form
solution
concentration
10 mg/mL in H2O
technique(s)
cell culture | mammalian: suitable
solubility
H2O: soluble 10 mg/mL
antibiotic activity spectrum
Gram-positive bacteria
neoplastics
parasites
Mode of action
protein synthesis | interferes
shipped in
wet ice
storage temp.
−20°C
SMILES string
Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1
InChI
1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1
InChI key
MKSVFGKWZLUTTO-FZFAUISWSA-N
Related Categories
1 of 4
This Item | P7255 | S8559 | 74667 |
|---|---|---|---|
| assay >98% (HPLC) | assay ≥98% (HPLC) | assay 95% (HPLC) | assay ≥85% (HPLC) |
| form solution | form powder | form powder | form solid |
| concentration 10 mg/mL in H2O | concentration - | concentration - | concentration - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) - |
| solubility H2O: soluble 10 mg/mL | solubility H2O: 50 mg/mL (Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.) | solubility H2O: complete 20 mg/ml, clear, colorless to light yellow, H2O: soluble | solubility H2O: soluble 200 mg/mL |
General description
Application
Biochem/physiol Actions
Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.
Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
Preparation Note
Other Notes
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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