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PHG0001

SAFC

HEPES

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
230-907-9
MDL number:
MFCD00006158
NACRES:
NA.21

biological source

synthetic

Quality Level

400

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

4.7-5.3 (, )

useful pH range

6.8-8.2

pKa (25 °C)

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
H4034H6147H3375
HEPES

SAFC

PHG0001

HEPES

HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

H4034

HEPES

HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

HEPES ≥99.5% (titration)

Sigma-Aldrich

H3375

HEPES

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | embryo: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

-

pH

4.7-5.3 (, )

pH

-

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (25 °C, 238 g/L)

suitability

suitable for manufacturing use

suitability

-

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

DNase, RNase, NICKase, protease, none detected

foreign activity

-

foreign activity

-

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.

HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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T1503
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

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