The concentration of this product varies from lot to lot and may range from 20 - 40 mg/mL. At the minimum concentration of 20 mg/mL, the 10 mg package size would have a volume of 0.5 mL. This information is published in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentation
R4642
Ribonuclease A from bovine pancreas
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
Synonym(s):
Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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10 MG
$47.60
50 MG
$164.00
250 MG
$505.00
1 G
$1,160.00
$47.60
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.53
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biological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt
13.7 kDa
~13,700
concentration
20-40 mg/mL
suitability
suitable for
foreign activity
Endonuclease and exonuclease, none detected
NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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This Item | R6513 | R5250 | R5500 |
|---|---|---|---|
| grade for molecular biology | grade for molecular biology | grade - | grade - |
| biological source bovine pancreas | biological source - | biological source bovine pancreas | biological source bovine pancreas |
| form (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) | form lyophilized | form buffered aqueous solution | form lyophilized powder |
| suitability suitable for | suitability - | suitability - | suitability suitable for mRNA or total RNA extracted from cells and tissues |
| concentration 20-40 mg/mL | concentration - | concentration - | concentration - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Features and Benefits
Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
Analysis Note
Protein determined by E.
Other Notes
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
related product
used together
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Wentao Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6752-6757 (2017-06-14)
Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, is the major cause of lung cancer. BaP forms covalent DNA adducts after metabolic activation and induces mutations. We have developed a method for capturing oligonucleotides carrying bulky base adducts, including UV-induced cyclobutane pyrimidine
Christopher P Selby et al.
The Journal of biological chemistry, 295(50), 17374-17380 (2020-10-23)
In nucleotide excision repair, bulky DNA lesions such as UV-induced cyclobutane pyrimidine dimers are removed from the genome by concerted dual incisions bracketing the lesion, followed by gap filling and ligation. So far, two dual-incision patterns have been discovered: the
Alessandro Ori et al.
Genome biology, 17, 47-47 (2016-03-16)
Recent large-scale studies revealed cell-type specific proteomes. However, protein complexes, the basic functional modules of a cell, have been so far mostly considered as static entities with well-defined structures. The co-expression of their members has not been systematically charted at
Netta Mäkinen et al.
PLoS genetics, 12(2), e1005850-e1005850 (2016-02-20)
Uterine leiomyosarcomas (ULMSs) are aggressive smooth muscle tumors associated with poor clinical outcome. Despite previous cytogenetic and molecular studies, their molecular background has remained elusive. To examine somatic variation in ULMS, we performed exome sequencing on 19 tumors. Altogether, 43
R Perdigão-Henriques et al.
Oncogene, 35(2), 158-172 (2015-03-24)
The miR-200 family promotes the epithelial state by suppressing the Zeb1/Zeb2 epithelial gene transcriptional repressors. To identify other miR-200-regulated genes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer cells. In all, 520 mRNAs were significantly enriched
Articles
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Protocols
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
Related Content
Instructions
Chromatograms
application for HPLC
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What is the Total Volume of this product?
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What is the actual concentration of 10MG R4642, Ribonuclease A in terms of mg/ml?
1 answer-
The mg/mL concentration of this product is lot dependent and reported in the product Certificate of Analysis. The specification for the concentration is 20 - 40 mg/mL. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/r4642#product-documentationPlease see the link below to review additional information is the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/984/r4642dat.pdfHelpful?
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Product R4642, Ribonuclease A from bovine pancreas (RNase A), is a solution. What does the solution contain?
1 answer-
The product is supplied in a solution containing 50% glycerol and 10 mM Tris-HCl, pH 8.0.
Helpful?
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When using Product R4642, Ribonuclease A from bovine pancreas (RNase A), do I need to boil the RNase A to inactivate residual DNases?
1 answer-
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity.
Helpful?
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Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
1 answer-
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the shelf life of Product R4642, Ribonuclease A from bovine pancreas (RNase A)?
1 answer-
This product is stable for at least 2 years when stored properly at -20 °C.
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