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R7755

SAFC

RPMI-1640 Medium

AutoMod™, without L-glutamine and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
R8755R1383R7638
SAFC

R7755

RPMI-1640 Medium

RPMI-1640 Medium Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

R8755

RPMI-1640 Medium

RPMI-1640 Medium With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

R1383

RPMI-1640 Medium

Sigma-Aldrich

R7638

RPMI-1640 Medium

form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
HEPES: no
NaHCO3: no
L-glutamine: no
phenol red: yes

components

HEPES: no
phenol red: no
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no

components

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

-

Quality Level

-

Quality Level

400

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Other Notes

Modified for autoclaving
Without L-glutamine and sodium bicarbonate.

Quantity

Formulated to contain 10.3 grams of powder per liter of medium.

Reconstitution

Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.

Legal Information

Auto-Mod is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000378788
0000378788
0000260213
SLCP5139
0000260213

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