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RAB0603

Sigma-Aldrich

Human ALB / Serum albumin ELISA Kit

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Synonym(s):
Serum Albumin
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 ng/mL
standard curve range: 4.915-1200 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... ALB(213)

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packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum
sample type urine

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 ng/mL
standard curve range: 4.915-1200 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20000 pg/mL
standard curve range: 31.25-2000 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.82-200 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.5 pg/mL
standard curve range: 1.64-400 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The ALB ELISA kit provides for the quantitative measurement of Albumin in Cell Culture Supernatants, Plasma and Serum.
This ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, cell culture supernatants, urine and/or cell and tissue lysates.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Human serum albumin (HSA) is a single, non-glycosylated chain that binds to various endogenous and exogenous ligands. It functions as a plasma transporter molecule and primarily binds to non-esterified long-chain fatty acids. Albumin also binds to and transports various metabolites, such as bilirubin, steroid hormones, thyroxin, tryptophan, certain vitamins and metal ions within the body. It also has the ability to bind to several drugs and affects their pharmacokinetics and pharmacodynamics. HSA functions as a nitric oxide-carrier and also influences the antioxidant capacity of human serum. In cases of acute hemolysis, HSA binds to heme in the blood stream and transports to hemopexin, where in it is reabsorbed by parenchymal liver cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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