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RES6003H-B7

SAFC

HEPES

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
NACRES:
NA.21

biological source

synthetic

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

4.7-5.3 (, )

useful pH range

6.8-8.2

pKa (25 °C)

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
PHG0001PHR1428H23830
HEPES

SAFC

RES6003H-B7

HEPES

-
HEPES

SAFC

PHG0001

HEPES

-
HEPES Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1428

HEPES

-
HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

Essential Grade
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

impurities

Endotoxin, microbial, and trace metals; tested

impurities

Endotoxin, microbial, and trace metals; tested

impurities

-

impurities

-

pH

4.7-5.3 (, )

pH

4.7-5.3 (, )

pH

5.0-6.5 (25 °C, 238 g/L)

pH

-

suitability

suitable for manufacturing use

suitability

suitable for manufacturing use

suitability

-

suitability

-

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

foreign activity

-

foreign activity

-

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Packaging

This product is available in the following package sizes:
RES6003H-B701X: 100g container
RES6003H-B703X: 5kg container
RES6003H-B705X: 25kg container
RES6003H-B706X: 50kg container

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

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HEPES sodium salt ≥99.0% (titration)

Sigma-Aldrich

H7006

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RES0197M-A7

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HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

H3784

HEPES sodium salt

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