Skip to Content
MilliporeSigma

S1452

Sigma-Aldrich

MISSION® siRNA Transfection Reagent

Synonym(s):

MISSION® siRNA Transfection Reagent

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

100 μL
$93.00
1 ML
$492.00

$93.00

Please contact Customer Service for Availability
Request a Bulk Order

About This Item

UNSPSC Code:
41106502
NACRES:
NA.51

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

product line

MISSION®

Quality Level

200

form

aqueous solution

shipped in

wet ice

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
71115SHC202VSITRAN-RO
MISSION® siRNA Transfection Reagent

Sigma-Aldrich

S1452

MISSION® siRNA Transfection Reagent

RiboJuice siRNA Transfection Reagent Efficient delivery of siRNA for targeted gene suppression

Sigma-Aldrich

71115

RiboJuice siRNA Transfection Reagent

MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Transduction Particles Targets no known mammalian genes

Sigma-Aldrich

SHC202V

MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Transduction Particles

X-tremeGENE™ siRNA Transfection Reagent Polymer reagent for delivering siRNA to common cell lines

Roche

SITRAN-RO

X-tremeGENE™ siRNA Transfection Reagent

product line

MISSION®

product line

-

product line

MISSION®

product line

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

form

aqueous solution

form

liquid

form

-

form

-

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

120.2 °F

Flash Point(C)

49 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCQ2525
SLCL7789
SLCJ4575
SLCJ2572
SLCG8596

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Tuyen T Dang et al.
Cancers, 12(7) (2020-07-11)
It was recently shown that the 5' to 3' exoribonuclease XRN2 is involved in the DNA damage response. Importantly, loss of XRN2 abrogates DNA double stranded break repair via the non-homologous end-joining pathway. However, the mechanistic details of how XRN2
Tuyen T Dang et al.
International journal of molecular sciences, 21(12) (2020-06-19)
Cellular survival is dependent on the efficient replication and transmission of genomic information. DNA damage can be introduced into the genome by several different methods, one being the act of DNA replication. Replication is a potent source of DNA damage
Ilaria Guerriero et al.
Oncotarget, 8(12), 19172-19191 (2016-11-24)
Hyperactivation of the PI3K/AKT pathway is observed in most human cancer including lung carcinomas. Here we have investigated the role of miRNAs as downstream targets of activated PI3K/AKT signaling in Non Small Cell Lung Cancer (NSCLC). To this aim, miRNA
A E Witt et al.
Oncogene, 36(12), 1707-1720 (2016-10-04)
Tumours are comprised of a highly heterogeneous population of cells, of which only a small subset of stem-like cells possess the ability to regenerate tumours in vivo. These cancer stem cells (CSCs) represent a significant clinical challenge as they are
Jose Miguel Escandell et al.
The EMBO journal, 38(7) (2019-02-24)
Telomeres, the protective ends of eukaryotic chromosomes, are replicated through concerted actions of conventional DNA polymerases and elongated by telomerase, but the regulation of this process is not fully understood. Telomere replication requires (Ctc1/Cdc13)-Stn1-Ten1, a telomeric ssDNA-binding complex homologous to
View All Related Papers

Articles

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Related Content

Transfection Reagent Selection Guide

Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.

Expression et extinction géniques

Sigma-Aldrich® Advanced Genomics est le premier fournisseur de technologies d'édition et d'extinction géniques, dont CRISPR, Cas9, les ARN guides synthétiques (ARNsg) et les nucléases à doigts de zinc (ZFN pour "Zinc Finger Nucleases").

Gene Expression & Silencing

Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).

Product Information Sheet - S1452

Instructions

See All

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service