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S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

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10 ML
$136.00
50 ML
$461.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.81

$136.00


Please contact Customer Service for Availability

biological source

Staphylococcus aureus

sterility

Not processed or packaged aseptically

form

buffered aqueous suspension

composition

Cell suspension, ~10% wet weight/volume

storage temp.

2-8°C

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This Item
P7155P603182526
biological source

Staphylococcus aureus

biological source

-

biological source

Staphylococcus aureus

biological source

Staphylococcus aureus

form

buffered aqueous suspension

form

cell suspension

form

essentially salt-free, lyophilized powder

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

sterility

Not processed or packaged aseptically

sterility

(not processed or packaged aseptically)

sterility

-

sterility

-

composition

Cell suspension, ~10% wet weight/volume

composition

-

composition

-

composition

-

General description

Staphylococcus aureus Wood 46 is protein A deficient and spa negative.[1] It shares 98% to 99% genome identity with S.aureus[1] and shows a lower surface expression of cell wall-associated protein A.[2]

Application

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck[3]
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva[4]
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt [5]

Wood 46, a non-protein A producing S. aureus strain[6], prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

Biochem/physiol Actions

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus.[1] This isolate is useful in understanding protein A role in pathogenesis and virulence.[2]

Physical form

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

Preparation Note

Produced in pure culture.

Analysis Note

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler[7].

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Questions

  1. Hello, I'm wondering if this "Staphylococcus aureus, wood 46 strain" is deactivated or not. Additionally, do you have the corresponding aptamer or antibody of this strain?

    1 answer
    1. This product is a formalin-fixed crude cell suspension of essentially non-viable S. aureus in 0.05 M potassium phosphate buffer containing 0.2% sodium azide. Currently, there is no corresponding aptamer or antibody for the Wood 46 Strain.

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