S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

Name: Streptavidin−Alkaline Phosphatase from <I>Streptomyces avidinii</I>
Brand: SIGMA
Price: 203 CAD

lyophilized powder, ≥700 DEA units/mg protein

Synonym(s):

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

Slide 1 of 1

Select a Size

250 μG

$203.00

1 MG

$543.00

$203.00

Please contact Customer Service for Availability

Request a Bulk Order

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00132384

Key Documents

View All Documentation

Product Name

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii, lyophilized powder, ≥700 DEA units/mg protein

conjugate

alkaline phosphatase conjugate

form

lyophilized powder

specific activity

≥700 DEA units/mg protein

composition

Protein, ≥40% biuret

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	S5512	189730	S4762
Sigma-Aldrich S2890 Streptavidin−Alkaline Phosphatase from Streptomyces avidinii	Sigma-Aldrich S5512 Streptavidin−Peroxidase from Streptomyces avidinii	Sigma-Aldrich 189730 Streptavidin	Sigma-Aldrich S4762 Streptavidin from Streptomyces avidinii
specific activity ≥700 DEA units/mg protein	specific activity -	specific activity ≥10 units/mg protein	specific activity ≥13 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized solid	form essentially salt-free, lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200
conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate	conjugate -	conjugate -
composition Protein, ≥40% biuret	composition Protein, ≥70% E1%/280	composition -	composition -

Application

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.[1][2][3]

The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin.[4] Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.[5]

Other Notes

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Physical form

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

SLCN4419

SLCL2931

SLCF8013

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Effects of bovine Herpesvirus Type 5 on development of in vitro-produced bovine embryos.

Silva-Frade C

Theriogenology, 73(3), 324-331 (2010)

Bystander activation of irrelevant CD4+ T cells following antigen-specific vaccination occurs in the presence and absence of adjuvant

van Aalst S, et al.

PLoS ONE, 12(5), e0177365-e0177365 (2017)

Sialoside specificity of the siglec family assessed using novel multivalent probes: identification of potent inhibitors of myelin-associated glycoprotein.

Ola Blixt et al.

The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)

Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using

Microbial Resource Conservation: Conventional to Modern Approaches (2018)

An allosteric-probe for detection of alkaline phosphatase activity and its application in immunoassay

Guo J, et al.

Frontiers in Chemistry, 6, 618-618 (2018)

View All Related Papers

Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii