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SAE0091

Sigma-Aldrich

Lysostaphin from Staphylococcus staphylolyticus

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein

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Synonym(s):
Lysostaphin from Staphylococcus staphylolyticus, Glycyl-glycine Endopeptidase
CAS Number:
Enzyme Commission number:
MDL number:

biological source

microbial (Staphylococcus staphylotyticus)

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

27 kDa

feature

DNA free

shipped in

wet ice

storage temp.

−20°C

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This Item
SRE0053L9043L7386
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

27 kDa

mol wt

26,926 Da

mol wt

-

mol wt

25 kDa

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

specific activity

≥500 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥3000 unit/mg solid

specific activity

≥500 units/mg protein

Application

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.
Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Physical form

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Ronald M Galiwango et al.
Microbiome, 10(1), 7-7 (2022-01-20)
Coronal sulcus (CS) anaerobe abundance and IL-8 levels are linked to HIV acquisition, and are dramatically reduced after penile circumcision (PC). The distal urethra may be the site of some HIV acquisition before PC, and presumably most acquisition post PC. We
Kojiro Tsujihana et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2116027119-e2116027119 (2022-06-16)
The epidermis is the outermost layer of the skin and the body's primary barrier to external pathogens; however, the early epidermal immune response remains to be mechanistically understood. We show that the chemokine CXCL14, produced by epidermal keratinocytes, exhibits robust
Judy J J Ou et al.
Frontiers in cellular and infection microbiology, 6, 187-187 (2017-01-14)
Background:Staphylococcus aureus (S. aureus) small colony variants (SCVs) can survive within the host intracellular milieu and are associated with chronic relapsing infections. However, it is unknown whether host invasion rates and immune responses differ between SCVs and their wild-type counterparts.
Astrid Haaskjold Lossius et al.
Dermatology (Basel, Switzerland), 238(1), 109-120 (2021-04-23)
The pathophysiology in atopic dermatitis (AD) is not fully understood, but immune dysfunction, skin barrier defects, and alterations of the skin microbiota are thought to play important roles. AD skin is frequently colonized with Staphylococcus aureus (S. aureus) and microbial
Caroline Meyer Olesen et al.
Microorganisms, 9(7) (2021-08-08)
Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin

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