SAE0092

Mutanolysin from Streptomyces globisporus ATCC 21553

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

• Synonym(s): Mutanolysin
• CAS Number: 55466-22-3
• MDL number: MFCD00131635
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: Streptomyces sp. (Streptomyces globisporus ATCC 21553)
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥4000 units/mg protein (biuret)
• mol wt: 23 kDa; ~18.5 kDa
• feature: DNA free
• concentration: ≥40% protein
• technique(s): nucleic acid detection: suitable
• suitability: suitable for cell lysis
• application(s): life science and biopharma
• shipped in: wet ice
• storage temp.: −20°C

Description

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),⁽⁸⁾ and also from other bacteria that are difficult to lyse with lysozyme only.

Biochem/physiol Actions

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Features and Benefits

Suitable for difficult-to-lyse samples.

Microbial DNA free, minimizing DNA contamination from reagents in workflow.

Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

Legal Information

Ficoll is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable