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SRE0007

Sigma-Aldrich

Mutanolysin from Streptomyces globisporus ATCC 21553

Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)

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Synonym(s):
Mutanolysin
CAS Number:
MDL number:
NACRES:
NA.54

biological source

bacterial (Streptomyces globisporus)

Quality Level

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

concentration

≥40% protein

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Legal Information

Ficoll is a registered trademark of Cytiva

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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