MilliporeSigma
All Photos(1)

SRE0015

Sigma-Aldrich

Salt Active Nuclease (SAN)

recombinant, expressed in Pichia pastoris

Synonym(s):
Endonucelase, Salt active endonuclease

recombinant

expressed in Pichia pastoris

Quality Level

form

buffered aqueous glycerol solution

specific activity

≥250000 U/mg

shipped in

wet ice

storage temp.

−20°C

Application

Salt active nuclease has been used to remove DNA during protein expression and purification.

Biochem/physiol Actions

Salt active nuclease (SAN) is a general, unspecific endonuclease that cleaves double-stranded and single-stranded DNA, and RNA. SAN is active at above neutral pH.  Unlike other nucleases, however, it has optimum activity at high concentrations of salt at high pH.  These qualities make SAN ideal for removal of DNA from cell extracts and protein samples.  The end-products upon complete degradation of DNA consist of a majority of 5 nucleotide
oligos.  The enzyme degrades DNA vs. RNA in a ratio of 10:1.

SAN is highly active over the temperature range 10-40 °C. The optimal NaCl-concentration for activity is 0.5 M.  However, SAN is active in low salt buffers.  1 M NaCl is not recommended for use of SAN.  Mg2+ (>1 mM) is required for activity.  The pH working range is above neutral pH, with an optimal range between pH 8.5-9.5

Physical form:  Solution in 25 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 0.5 M NaCl, 0.01% Triton, and 50% (v/v) glycerol

Unit definition:  One unit (U) is defined as an increase in absorbance at 260 nm of 1 Absorbance Unit in 30 minutes at 37 ºC, using 50 μg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 ºC), 5 mM MgCl2, and 500 mM NaCl.

Unit Definition

One unit (U) is defined as an increase in absorbance at 260 nm of 1 absorbance unit (AU) in 30 minutes at 37 °C, using 50 μg/ml calf thymus DNA in a buffer with 25 mM Tris-HCl (pH 8.5, 25 °C), 5 mM MgCl2, and 500 mM NaCl.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service