SRP3058

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SRP3058

IFN-γ human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

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Synonym(s):

Immune Interferon, MAF, T cell interferon, type II interferon

NACRES:

NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

5.0-10.0 ng/mL ED₅₀

mol wt

16.7 kDa

packaging

pkg of 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P01579

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IFNG(3458)

Related Categories

Animal Component Free Cell Culture Reagents

Growth Factors and Cytokines

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This Item	SRP6291	SRP6222	SRP6221
Sigma-Aldrich SRP3058 IFN-γ human	Sigma-Aldrich SRP6291 IFN-ω human	Sigma-Aldrich SRP6222 IFN-γ2 human	Sigma-Aldrich SRP6221 IFN-γ1 human
biological source human	biological source human	biological source human	biological source human
recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in E. coli	recombinant expressed in E. coli
assay ≥98% (HPLC), ≥98% (SDS-PAGE)	assay ≥93% (SDS-PAGE)	assay ≥98% (SDS-PAGE)	assay ≥98% (SDS-PAGE)
form lyophilized	form lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 16.7 kDa	mol wt calculated mol wt 16.8 kDa (no tag), observed mol wt 25.0 kDa (DTT-reduced. Protein migrates due to glycosylation. Gln 24 is the predicted N-terminus.)	mol wt 19.6 kDa	mol wt 19.8 kDa
packaging pkg of 100 μg	packaging pkg of 10 μg	packaging pkg of 10 and 50 μg	packaging pkg of 10 and 50 μg

General description

IFN-γ (interferon γ) is an acid-labile glycosylated interferon produced by CD4 and CD8 T lymphocytes as well as activated NK (natural killer) cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC (major histocompatibility complex) proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. The gene is mapped to human chromosome 12q15. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.7kDa protein containing 143 amino acid residues.

Application

IFN-γ (interferon γ)-human has been used for the generation of inflammatory M1 cells from human monocytic cell line, THP1.

Biochem/physiol Actions

IFN-γ (interferon γ) signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. It can suppress the generation of anti-inflammatory cytokines and enhance the secretion of proinflammatory cytokines. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK (natural killer) cells and neutrophils. It is responsible for tumor rejection and is capable of killing tumor cells by autophagy or apoptosis. IFN-γ is also involved in Hepatitis E virus disease pathogenesis.

Sequence

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.4.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)