MilliporeSigma
All Photos(1)

Documents

SRP3073

Sigma-Aldrich

IL-12 human

recombinant, expressed in CHO cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
CTL maturation factor (TCMF),, Cytotoxic lymphocyte maturation factor (CLMF), NKSF, TSF

biological source

human

recombinant

expressed in CHO cells

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤1.0 ng/mL ED50

mol wt

40 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL12A(3592)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
I2276SRP3078SRP3075
Sigma-Aldrich

Sigma-Aldrich

SRP3073

IL-12 human

Sigma-Aldrich

Sigma-Aldrich

I2276

Interleukin-12 human

Sigma-Aldrich

Sigma-Aldrich

SRP3078

IL-16 (121aa) human

Sigma-Aldrich

Sigma-Aldrich

SRP3075

IL-12p80 human

recombinant

expressed in CHO cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

recombinant

expressed in (BTI-Tn-5B1-4) High-5 Insect Cells

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

lyophilized

form

lyophilized

mol wt

40 kDa

mol wt

glycoprotein 75 kDa

mol wt

12.4 kDa

mol wt

80.0 kDa

packaging

pkg of 10 μg

packaging

pkg of 5 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

General description

IL-12 (interleukin 12) is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue type mast cells. Recombinant Human IL-12 is a 75kDa heterodimeric glycoprotein consisting of disulfide-linked 35kDa (p35) and 40kDa (p40) subunits (503 total amino acid residues).

Biochem/physiol Actions

IL-12 (interleukin 12) is a proinflammatory cytokine and plays an important role in protection against pathogens. IL-12 is a potent regulator of cell mediated immune responses and it induces IFN (interferon)-γ production by NK (natural killer) and T cells. Among its biological activities IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells and induces the development of IFN-γ producing Th1 (T helper) cells. Polymorphisms in the IL-12 gene are associated with risk of rheumatoid arthritis. It also exhibits anti-tumor activities by enhancing Th1 adaptive immunity and cytotoxic T lymphocyte functions.

Sequence

p35 Subunit: RNLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNAS p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Physical form

Lyophilized from 0.5x PBS, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2-7.4 to a concentration of 0.2 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

Sigma-Aldrich

Sigma-Aldrich

I2644

Interleukin-2 human

PMA for use in molecular biology applications, &#8805;99% (HPLC)

Sigma-Aldrich

P1585

PMA

An absolute and restricted requirement for IL-12 in natural killer cell IFN-gamma production and antiviral defense. Studies of natural killer and T cell responses in contrasting viral infections.
Orange JS and Biron CA
Journal of Immunology, 156, 1138-1142 (1996)
Up-regulation of IL-12 expression in patients with chronic hepatitis B is mediated by the PI3K/Akt pathway.
Wang HW, te al.
Molecular and Cellular Biochemistry, 407, 135-142 (2015)
IL12 polymorphisms, HBV infection and risk of hepatocellular carcinoma in a high-risk Chinese population.
Liu L, et al.
International Journal of Cancer. Journal International Du Cancer, 128, 1692-1696 (2011)
IL-12 induces the production of IFN-gamma by neonatal human CD4 T cells.
Wu CY, et al.
Journal of Immunology, 151, 1938-1949 (1993)
Martin J Page et al.
British journal of haematology, 180(1), 110-117 (2017-11-17)
Inflammation, with its associated inflammatory molecules, is integral to most chronic diseases, including the various cardiovascular diseases. Interleukin 12 (IL12) is one of the inflammatory cytokines that is upregulated during inflammation; however, we know very little about its exact effect

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service