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SRP3085

Sigma-Aldrich

Interleukin-2, human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

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Synonym(s):
Aldesleukin, IL-2, T-cell growth factor (TCGF)
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥95% (HPLC)

form

(Lyophilized with no additives from a 0.2µ filtered solution)

potency

≤0.5 ng/mL ED50

mol wt

15.5 kDa

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL2(3558)

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recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in CHO cells

assay

≥95% (HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

(Lyophilized with no additives from a 0.2µ filtered solution)

form

lyophilized

form

lyophilized

form

lyophilized

mol wt

15.5 kDa

mol wt

15.0 kDa

mol wt

15.4 kDa

mol wt

40 kDa

packaging

pkg of 50 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

General description

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. The gene is mapped to human chromosome 4q27. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Application

  • IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform.
  • It has been used for the production of tumor-specific cytotoxic T lymphocytes.
  • It has been used for the proliferation of T lymphocytes.

Biochem/physiol Actions

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.

Sequence

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

Physical form

Lyophilized with no additives.

Reconstitution

Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 1 week. For extended storage, freeze in working aliquots. It may be advisable to centrifuge the vial prior to reconstitution.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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Protocols

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

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