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SRP3312

Sigma-Aldrich

IL-10 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

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Synonym(s):
B-TCGF, CSIF, TGIF
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL

mol wt

18.6 kDa

packaging

pkg of 10 μg

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL10(3586)

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SRP3312

IL-10 human

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SRP3071

IL-10 human

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SRP3072

IL-11 human

Sigma-Aldrich

Sigma-Aldrich

SRP3079

IL-16 human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

mol wt

18.6 kDa

mol wt

18.6 kDa

mol wt

19.1 kDa

mol wt

12.4 kDa

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

General description

Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. The gene encoding this protein is localized on human chromosome 1q31-32. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Interleukin 10 (IL-10) comprises interferon IFN-γ fold and exists as a symmetric homodimer. It shares structural similarity with interferon γ (IFN-γ).

Application

Interleukin 10 (IL-10) human has been used in enzyme-linked immunosorbent assay.

Biochem/physiol Actions

Interleukin-10 (IL-10) is an anti-inflammatory protein that inhibits the expression of proinflammatory cytokines such as IL-1 and tumor necrosis factor-α (TNF-α). Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. It inhibits the production of IFN-γ and antigen-specific proliferation of type-1 T helper cells (Th1). It protects the host tissue from damage during immune responses. The protein has a role in modulating viral immunity. IL-10 enhances cytokine production, natural killer cell proliferation and cytotoxicity in vitro. IL-10 is an immunosuppressive cytokine. It promotes wound repair and functions to resolve inflammation in the central nervous system. IL-10 mediates its signaling function through the interleukin receptors namely, IL-10RA and two IL-10RBs. The expression of IL-10 is regulated by signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling-3 (SOCS3) ratios. Polymorphisms in the IL-10 gene is implicated in breast cancer.

Physical form

Lyophilized from 20 mM Sodium Phosphate, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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