SRP6170

IL-2 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): Aldesleukin, Lymphokine, N/A, T-cell growth factor (TCGF)
• CAS Number: 110942-02-4
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 0.2-2 ng/mL
• mol wt: 15 kDa (monomer, glycosylated)
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• NCBI accession no.: P60568
• UniProt accession no.: P60568
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... IL2(3558)

Description

General description

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

The IL-2 gene is mapped to human chromosome 4q27.

Application

IL-2 (interleukin 2) human has been used for the stimulation of human primary naive T lymphocytes to study the effect of microRNA-146a on IL-2 activation-induced cell death.

Biochem/physiol Actions

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL-2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.

Physical form

Lyophilized from a PBS solution.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable