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STRN250

Sigma-Aldrich

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 250 purifications

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Synonym(s):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
NACRES:
NA.52

usage

sufficient for 250 purifications

Quality Level

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

RNA purification: suitable

greener alternative category

storage temp.

15-25°C

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This Item
STRN50STRN10RTN350
usage

sufficient for 250 purifications

usage

sufficient for 50 purifications

usage

sufficient for 10 purifications

usage

sufficient for 350 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

STRN10

STRN10, STRN250

STRN250, STRN50

RTN10, RTN70, RTN9602, RTN9604

greener alternative category

Aligned,

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

Aligned,

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

General description

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Application

Spectrum®Plant Total RNA Kit has been used:
  • to extract RNA from the material ground in liquid nitrogen
  • to isolate total RNA from mutant and wt (wild type) plantlets
  • to extract total RNA from leaf samples
  • to extract RNA from all plant tissues

Features and Benefits

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Legal Information

Specac is a registered trademark of Specac Ltd.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3


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Related Content

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service