This material may be stored in solution for up to 1 year at -20°C. Please see the link below to review product T7659, the prepared 1X Trypsin Inhibitor Solution in DPBS:
https://www.sigmaaldrich.com/product/sigma/t7659
T9128
Trypsin inhibitor
lyophilized powder, suitable for inhibition assay
Synonym(s):
SBTI
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500 MG
$205.00
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$336.00
5 G
$1,200.00
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$1,980.00
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500 MG
$205.00
1 G
$336.00
5 G
$1,200.00
10 G
$1,980.00
About This Item
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Product Name
Trypsin inhibitor from Glycine max (soybean), lyophilized powder
biological source
Glycine max (soybean)
type
Type II-S
form
lyophilized powder
mol wt
20,100 Da
technique(s)
inhibition assay: suitable
solubility
balanced salt solution: 1 mg/mL
concentrate: >10 mg/mL, hazy, amber-yellow
phosphate buffer: 10 mg/mL
water: 10 mg/mL
serum-free medium: soluble
shipped in
ambient
storage temp.
2-8°C
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1 of 4
This Item | 93620 | T6522 | T2327 |
|---|---|---|---|
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 300 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form powder | form lyophilized powder |
| shipped in ambient | shipped in wet ice | shipped in ambient | shipped in - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility balanced salt solution: 1 mg/mL, phosphate buffer: 10 mg/mL, serum-free medium: soluble, concentrate: >10 mg/mL, hazy, amber-yellow, water: 10 mg/mL | solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless | solubility balanced salt solution: 1 mg/mL, serum-free medium: soluble | solubility - |
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Components
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
Unit Definition
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Analysis Note
One mg of trypsin inhibitor will inhibit a minimum of 1.0 mg Trypsin of activity ~10,000 BAEE units/mg trypsin.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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how long can STI solution be stable in -20degC?
1 answer-
Helpful?
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Is this product suitable for cell culture
1 answer-
This product has not been tested for use in cell culture. However, this item has been used in cell culture applications by other researchers. Please see the link below to review one example:
https://pmc.ncbi.nlm.nih.gov/articles/PMC4138974/
Please see the links below to review two cell culture tested product options currently available:
T6522, powder, BioReagent, suitable for cell culture
https://www.sigmaaldrich.com/product/sigma/t6522
T6414, solution, sterile-filtered, suitable for, suitable for cell culture
https://www.sigmaaldrich.com/product/sigma/t6414Helpful?
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
1 answer-
Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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