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MilliporeSigma

T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Synonym(s):

Tris-EDTA wash buffer, TE buffer solution

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100 ML

$51.00

$51.00

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About This Item

NACRES:
NA.25
PubChem Substance ID:
329826904
UNSPSC Code:
41105319
MDL number:
MFCD00236359

Key Documents

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InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

Quality Level

200

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This Item
932839330293290
Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting

Sigma-Aldrich

T9285

Tris-EDTA buffer solution

Tris-EDTA buffer solution BioUltra, Molecular Biology, pH 8.0

Sigma-Aldrich

93283

Tris-EDTA buffer solution

Tris-EDTA buffer solution BioUltra, Molecular Biology, pH 7.4

Supelco

93302

Tris-EDTA buffer solution

TRIS borate – EDTA buffer solution for Northern and Southern blotting, solution

Sigma-Aldrich

93290

TRIS borate – EDTA buffer solution

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

form

liquid

form

liquid

form

liquid

form

solution

application(s)

sample preparation

application(s)

sample preparation

application(s)

-

application(s)

-

pH

7.8-8.2 (1 × in water)

pH

8.0±0.2 (25 °C)

pH

7.4±0.2 (25 °C)

pH

8.6-9.0

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR[1][2]
  • gel electrophoresis, including Northern and Southern blotting[3]
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells[4]
  • antigen retrieval for immunohistochemistry[3]

Preparation Note

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000391230
0000386778
0000386778
SLCN5931
SLCN5931

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Shiraz Tyebji et al.
Cell reports, 31(4), 107573-107573 (2020-04-30)
Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)
Kashif Asghar et al.
Experimental and therapeutic medicine, 9(3), 901-904 (2015-02-11)
Indoleamine 2,3-dioxygenase (IDO) is an immunoregulatory enzyme. It plays a key role in various malignancies, infection and autoimmune diseases. IDO induces immunosuppression through the depletion of tryptophan and its downstream metabolites. Hepatitis C virus (HCV) has infected more than 12
Jennifer A Black et al.
Journal of clinical microbiology, 43(7), 3110-3113 (2005-07-08)
Although plasmid-mediated AmpC beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. These enzymes are typically produced by isolates of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. and are
Virtual Fluorescence Color Channels by Selective Photobleaching in Digital PCR Applied to the Quantification of KRAS Point Mutations
Schlenker F et al.
Analytical Chemistry, 93, 10538-10545 (2021)
View All Related Papers

Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. What is the molarity of the 100X Tris EDTA solution?

    1 answer

    1. At the 100X concentration this solution contains 1M Tris-HCl and 0.1M EDTA. When diluted to 1X (1:100), the solution is contains 10mM Tris-HCL and 1mM EDTA.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Can this Tris-EDTA buffer solution be used in an IHC protocol for antigen retrieval?

    1 answer

    1. This product has not been tested specifically for antigen retrieval. The researcher would have to determine suitability. Please see the link below to product E1161. This product is a prepared 10X solution formulated specifically for retrieval and may be better suited the specified application:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/855/e1161pis.pdf

      Helpful?

  5. What does 'for molecular biology' mean?

    1 answer

    1. The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the shelf life of Product T9285, Tris-EDTA buffer solution?

    1 answer

    1. This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.

      Helpful?

  8. What is the pH and color of Product T9285, Tris-EDTA buffer solution?

    1 answer

    1. The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.

      Helpful?

  9. What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?

    1 answer

    1. When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.

      Helpful?

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