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MilliporeSigma

T9378

Sigma-Aldrich

Trypsin inhibitor

crude powder

Synonym(s):

Trypsin Inhibitor from lima beans

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100 MG
$232.00
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$842.00
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$1,390.00

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100 MG
$232.00
500 MG
$842.00
1 G
$1,390.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

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$232.00


Please contact Customer Service for Availability

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Product Name

Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder

biological source

Phaseolus limensis (lima bean)

Quality Level

type

Type II-L

form

crude powder

mol wt

9 kDa

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

storage temp.

2-8°C

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This Item
93620T6522T9003
biological source

Phaseolus limensis (lima bean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

biological source

Glycine max (soybean)

Quality Level

200

Quality Level

100

Quality Level

300

Quality Level

300

form

crude powder

form

lyophilized powder

form

powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

solubility

0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless

solubility

balanced salt solution: 1 mg/mL, serum-free medium: soluble

solubility

H2O: >10 mg/mL, phosphate buffer: >10 mg/mL (hazy above 10 mg/ml), soluble

mol wt

9 kDa

mol wt

Mr ~20000

mol wt

20.1 kDa

mol wt

20.1 kDa

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
  • in the inhibition of trypsin in human plasma[1]
  • in the termination of proteolytic digestion in rabbit skeletal muscles[2]
  • in the inhibition of salivary glands enzyme extract from Lygus lineolaris[3]
  • in the inhibition of proteolytic activity in Agave tequilana enzyme extract[4]

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type[5] enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors.[6] It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.[7]

Physical properties

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Unit Definition

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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