T9777

Trypsin-chymotrypsin inhibitor

lyophilized powder

• Synonym(s): Bowman-Birk Inhibitor
• CAS Number: 37330-34-0
• MDL number: MFCD00132532
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• Product Name: Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder
• biological source: Glycine max (soybean)
• Quality Level: 200
• form: lyophilized powder
• composition: Protein, 70-90% biuret
• storage temp.: 2-8°C

Description

General description

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops.[1] Trypsin-chymotrypsin has anticarcinogenic properties.[2]

Application

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.

Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay.[3] It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.[4]

Unit Definition

One trypsin unit = ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Physical form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Analysis Note

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves