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Western Blocker Solution

for HRP detection systems

Western blot blocking solution, blocking buffer

Quality Level

shelf life

≥2 yr

storage temp.


General description

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.


Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes.[1][2][3] It has also been used for dilution of antibodies in western blot.[4]

Features and Benefits

  • Does not inhibit weak HRP signals, as many milk-based blockers do.
  • Can be used both as a blocking component and as a probing component.
  • Supplied ready-to-use; no preparative step is required.

Legal Information

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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