Z2126

ZAP-70 Tyrosine Kinase human

Name: ZAP-70 Tyrosine Kinase human
Brand: SIGMA
Price: 1270 CAD

≥90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells (Histidine tagged), buffered aqueous glycerol solution, ≥150 U/mg

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20 μG

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About This Item

MDL number:

MFCD03459122

UNSPSC Code:

12352200

NACRES:

NA.32

Key Documents

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recombinant

expressed in baculovirus infected insect cells (Histidine tagged)

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥150 U/mg

mol wt

70 kDa

UniProt accession no.

P43403

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... ZAP70(7535)

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This Item	SAB4504011	SAB4504012	WH0007535M1
Sigma-Aldrich Z2126 ZAP-70 Tyrosine Kinase human	Sigma-Aldrich SAB4504011 Anti-phospho-ZAP-70 (pTyr²⁹²) antibody produced in rabbit	Sigma-Aldrich SAB4504012 Anti-phospho-ZAP-70 (pTyr³¹⁵) antibody produced in rabbit	Sigma-Aldrich WH0007535M1 Monoclonal Anti-ZAP70 antibody produced in mouse
Gene Information human ... ZAP70(7535)	Gene Information human ... ZAP70(7535)	Gene Information human ... ZAP70(7535)	Gene Information human ... ZAP70(7535)
assay ≥90% (SDS-PAGE)	assay -	assay -	assay -
specific activity ≥150 U/mg	specific activity -	specific activity -	specific activity -
form buffered aqueous glycerol solution	form buffered aqueous solution	form buffered aqueous solution	form buffered aqueous solution
recombinant expressed in baculovirus infected insect cells (Histidine tagged)	recombinant -	recombinant -	recombinant -
mol wt 70 kDa	mol wt antigen 69 kDa	mol wt antigen 69 kDa	mol wt -

Application

Human ZAP-70 Tyrosine Kinase was used for mapping the phosphorylation sites on LAT (linker for activation of T cells) for investigating the mechanism of activation of signalling proteins in T cells.[1]

Biochem/physiol Actions

ZAP-70 Tyrosine Kinase is a protein belongs to Syk family and is essential for T cell activation.[2] It facilitates the upregulation of Fas ligand in activation-induced T cell apoptosis.[3] Additionally, it also plays a pivotal role in LFA-1-dependent T Cell Migration.[4]

ZAP-70 is protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells. It is required for T cell activation. It is activated by Lck-mediated phosphorylation of its tyrosine residues.

Physical form

Solution in 25 mM HEPES, pH 7.5, containing 150 mM NaCl, 0.5 mM DTT, and 50% glycerol.

Other Notes

One unit will phosphorylate 1 nmol polyglutamic acid:tyrosine per min at 37 °C at pH 7.0.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

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ZAP-70 tyrosine kinase is required for the up-regulation of Fas ligand in activation-induced T cell apoptosis.

C M Eischen et al.

Journal of immunology (Baltimore, Md. : 1950), 159(3), 1135-1139 (1997-08-01)

Activation-induced cell death (AICD) is initiated by the TCR-dependent up-regulation of Fas ligand (FasL) mRNA. The subsequently generated soluble or cell-associated FasL gene products bind Fas, leading to apoptosis of the T cells. Although TCR stimulation is essential to initiate

ZAP-70 tyrosine kinase is required for LFA-1-dependent T cell migration.

R D Soede et al.

The Journal of cell biology, 142(5), 1371-1379 (1998-09-10)

The ZAP-70 tyrosine kinase is essential for T cell activation by the T cell receptor. We show that ZAP-70 is also required for migration of T cells that is dependent on the integrin LFA-1. Invasion of TAM2D2 T cell hybridoma

Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells.

P E Paz et al.

The Biochemical journal, 356(Pt 2), 461-471 (2001-05-23)

T-cell-receptor (TCR)-mediated LAT (linker for activation of T cells) phosphorylation is critical for the membrane recruitment of signalling complexes required for T-cell activation. Although tyrosine phosphorylation of LAT is required for recruitment and activation of signalling proteins, the molecular mechanism

The Syk family of protein tyrosine kinases in T-cell activation and development.

D H Chu et al.

Immunological reviews, 165, 167-180 (1998-12-16)

The processes of T-cell development and activation employ similar immature and mature receptors as well as similar signal transduction pathways to achieve different outcomes. Many signaling molecules are shared between the receptor signaling pathways, including two families of cytoplasmic protein

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ZAP-70 Tyrosine Kinase human