Plant Culture Media
Growing plant tissue in the laboratory may include seed, meristem, callus, and bud culture, and requires specialized plant culture media. Murashige and Skoog (also called MS media, MSO, or MS0) and Gamborg’s B5 medium are two of the most essential media formulations used for culturing plants. These media contain all the micro- and macronutrients, vitamins, organic supplements, and plant growth regulators that are necessary for the growth and multiplication of plant cells, tissues, and organs in vitro. For applications that require more custom formulations, we offer a comprehensive selection of basal salt mixtures, vitamin solutions, and supplements.
Products
D-(+)-Glucose
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Dimethyl sulfoxide
≥99.5% (GC), suitable for plant cell culture
Agar
microbiology tested, suitable for plant cell culture, suitable for cell culture, powder
Potassium phosphate monobasic
powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Biotin
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Kanamycin sulfate from Streptomyces kanamyceticus
powder, BioReagent, suitable for cell culture, suitable for plant cell culture
L-Ascorbic acid
powder, suitable for cell culture, γ-irradiated
Sodium chloride
BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Phytagel™
BioReagent, suitable for plant cell culture, powder
Murashige and Skoog Basal Medium
powder, suitable for plant cell culture
L-Ascorbic acid
suitable for cell culture, suitable for plant cell culture, ≥98%
β-Glycerophosphate disodium salt hydrate
BioUltra, suitable for cell culture, suitable for plant cell culture, ≥99% (titration)
Boric acid
BioReagent, Molecular Biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
Murashige and Skoog Basal Salt Mixture (MS)
powder, suitable for plant cell culture
Calcium chloride
anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Calcium chloride dihydrate
BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Gibberellic acid
BioReagent, suitable for plant cell culture, ≥90% gibberellin A3 basis (of total gibberellins.)
myo-Inositol
≥99% (GC), BioReagent
6-Benzylaminopurine
suitable for plant cell culture
Cyanobacteria BG-11 Freshwater Solution
50 ×, liquid, suitable for plant cell culture
Murashige and Skoog (MS Media)
Much of plant tissue culture for research and biotechnology still relies on media described more than 50 years ago by Murashige and Skoog. Their eponymous Murashige and Skoog media, or MS media, is the most widely-used plant culture medium and is available as basal salt mixtures or media containing organics.
Regardless of format, MS media is comprised of micro- and macronutrients, plus nutrients like sugar, vitamins, and growth regulators. Different MS media components can be custom-combined to create the right media formulation for each plant cell or tissue type.
Gamborg’s B5 Medium
Gamborg′s B5 medium is an optimized culture medium for in vitro plant cell, tissue, and organ culture. It contains a mixture of inorganic salts, vitamins, and carbohydrates. We offer Gamborg’s B5 medium with minimal organics, Gamborg’s vitamin mix, and Gamborg’s B5 basal salt mix.
Phytamax™ Orchid Culture Medium
Phytamax™ Orchid Multiplication Medium and Phytamax™ Orchid Maintenance Medium are used for culturing, propagating and replating the orchid stem. Phytamax™ orchid culture media are available with or without activated charcoal, and with both charcoal and banana powder.
Related Resources
- Article: Antibiotics in Plant Tissue Culture Protocol
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
- Article: Classical Plant Media Formulations
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
- Article: Explant Sterilization – Plant Tissue Culture Protocol
Surface sterilizing plant material is an important for plant tissue culture. Commonly used disinfectants for plant tissue culture and methods for disinfecting seeds are included.
- Article: Growth Regulators – Plant Tissue Culture Protocol
Use the tables in this protocol to assist in the preparation of auxins, cytokins, and other regulators for use in plant cell culture media.
- Article: Media Preparation – Plant Tissue Culture Protocol
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
- Article: Pathology Media – Plant Tissue Culture Protocol
Fungal and Bacterial Detection Media, Media Preparation and Storage, Media Composition Table.
- Article: Phycology Media in Plant Tissue Culture
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
- Brochure: Plant Tissue Culture Products to Ensure Your Success
Sigma-Aldrich’s combination of qualified plant tissue culture products and global business capabilities help customers maintain reagent consistency and suitability.
- Article: Silver Thiosulfate – Plant Tissue Culture Protocol
Preparation instructions for 0.1 M Sodium Thiosulfate (STS) and 0.1 M Silver Nitrate stock solutions for laboratory use.
- Article: Vitamin Mixtures – Plant Tissue Culture Protocol
Preparation of Solutions from Packaged Powder, Media Preparation using Vitamin Solutions, Vitamin Mixtures Composition Table
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