Phosphatase and protease inhibitor cocktails are used to prevent proteolysis and dephosphorylation of proteins during extraction, sample preparation, and analysis. A variety of phosphatase and protease inhibitor cocktails as well as individual inhibitors are available to prevent sample degradation and preserve the native state of proteins during sample preparation. Our inhibitor cocktails are designed and optimized for specific tissues including mammalian, plant, bacterial, and yeast.
During cell signaling, target proteins are phosphorylated by protein kinases that transfer a phosphate group to specific sites and are removed by endogenous protein phosphatases upon cell lysis. Phosphatase inhibitors help preserve the activity and state of phosphoproteins during cell lysis and protein extraction. Phosphatase inhibitors are tailored for broad-spectrum use, provide protection against specific classes of phosphatases, and are suitable for use with cell lysates and tissue extracts, including samples containing detergents.
During protein expression and isolation, endogenous proteases rapidly degrade protein samples, reducing the quality and quantity of your protein samples required for characterization and analysis. Protease inhibitor cocktails and highly specific protease inhibitors ensure protein integrity during protein extraction and preparation. Protease inhibitors are suitable for numerous sample types, including bacterial cell extracts, mammalian cells, tissue extracts, plant cells, tissue extracts, yeast, and fungal extract. Protect your protein preparations from common proteases, including serine proteases, metalloproteases, cysteine proteases, aminopeptidases, and aspartic proteases.
ReadyShield® inhibitor cocktails are formulated to be non-freezing for direct use from the freezer. In addition to this ready-to-use format, ReadyShield® inhibitor cocktails also deliver the following features and options:
To learn more about our ReadyShield® Phosphatase and Protease Inhibitor Cocktails, explore our ReadyShield® FAQ page, which includes a convenient selection table to help you choose the right ReadyShield® product for your workflow needs.
Roche offers a broad selection of convenient protease inhibitor cocktails as well as optimized lysis reagents to ensure maximum yields of intact and functional proteins.