Phosphatase & Protease Inhibitors
Phosphatase and protease inhibitor cocktails are used to prevent proteolysis and dephosphorylation of proteins during extraction, sample preparation, and analysis. A variety of phosphatase and protease inhibitor cocktails as well as individual inhibitors are available to prevent sample degradation and preserve the native state of proteins during sample preparation. Our inhibitor cocktails are designed and optimized for specific tissues including mammalian, plant, bacterial, and yeast.
Products
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail
Protease Inhibitor Cocktail Tablets provided in a glass vial, Tablets provided in a glass vial
PhosSTOP™
sufficient for 10 10mL buffer preparations, sufficient for 20 10mL buffer preparations, suitable for tissue processing, suitable for immunoprecipitation (IP)
cOmplete™, EDTA-free Protease Inhibitor Cocktail
Tablets provided in glass vials
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail
Tablets provided in EASYpacks
cOmplete™ Protease Inhibitor Cocktail
Tablets provided in glass vials
cOmplete™, EDTA-free Protease Inhibitor Cocktail
Tablets provided in EASYpacks
cOmplete™, Mini Protease Inhibitor Cocktail
Tablets provided in a glass vial
Phosphatase Inhibitor Cocktail 3
DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution
Phosphatase Inhibitor Cocktail 2
aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases
cOmplete™ ULTRA Tablets, Mini, EDTA-free, EASYpack Protease Inhibitor Cocktail
Tablets supplied in foil blister packs.
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder
cOmplete™ Protease Inhibitor Cocktail
Tablets provided in EASYpacks
cOmplete™, Mini Protease Inhibitor Cocktail
Tablets provided in EASYpacks
Protease Inhibitor Cocktail
SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins
cOmplete™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail
Tablets supplied in foil blister packs.
cOmplete™ ULTRA Tablets, EDTA-free, glass vials Protease Inhibitor Cocktail
Tablets provided in glass vials
Protease Inhibitor Cocktail III
EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution
Protease and Phosphatase Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase
PHOSPHATASE AND PROTEASE INHIBITOR COCKTAILS
During cell signaling, target proteins are phosphorylated by protein kinases that transfer a phosphate group to specific sites and are removed by endogenous protein phosphatases upon cell lysis. Phosphatase inhibitors help preserve the activity and state of phosphoproteins during cell lysis and protein extraction. Phosphatase inhibitors are tailored for broad-spectrum use, provide protection against specific classes of phosphatases, and are suitable for use with cell lysates and tissue extracts, including samples containing detergents.
During protein expression and isolation, endogenous proteases rapidly degrade protein samples, reducing the quality and quantity of your protein samples required for characterization and analysis. Protease inhibitor cocktails and highly specific protease inhibitors ensure protein integrity during protein extraction and preparation. Protease inhibitors are suitable for numerous sample types, including bacterial cell extracts, mammalian cells, tissue extracts, plant cells, tissue extracts, yeast, and fungal extract. Protect your protein preparations from common proteases, including serine proteases, metalloproteases, cysteine proteases, aminopeptidases, and aspartic proteases.
ReadyShield® Phosphatase and Protease Inhibitor Cocktails
ReadyShield® inhibitor cocktails are formulated to be non-freezing for direct use from the freezer. In addition to this ready-to-use format, ReadyShield® inhibitor cocktails also deliver the following features and options:
- Protease, phosphatase, or combined cocktails available
- Tailored for use for a variety of sample types
- Transparency as to which inhibitors are included in each cocktail
To learn more about our ReadyShield® Phosphatase and Protease Inhibitor Cocktails, explore our ReadyShield® FAQ page, which includes a convenient selection table to help you choose the right ReadyShield® product for your workflow needs.
Roche cOmplete™ Inhibitor Cocktails
Roche offers a broad selection of convenient protease inhibitor cocktails as well as optimized lysis reagents to ensure maximum yields of intact and functional proteins.
- Inhibit proteolytic activity in extracts from almost any tissue or cell type, including animals, plants, yeast, bacteria, and fungi
- Convenient quick-dissolving tablets can be added to lysis buffer without weighing
- Classic cOmplete™ Tablets from Roche for routine protein extraction and analysis
- PhosSTOP™ Phosphatase Inhibitor Cocktail Tablets for inhibition of a broad spectrum of phosphatases across a wide range of samples (mammalian, plant, yeast, and bacteria)
- cOmplete™ ULTRA or cOmplete™ Tablets with PhosSTOP™ Tablets for comprehensive protection against both proteases and phosphatases
Related Resources
- Brochure: Refine Protein Preparation - Tools for Better Protein Analysis
With over 50 years of experience in developing protein sample preparation technologies, we are constantly innovating new tools to offer you rapid and efficient solutions that can be smoothly integrated into your workflow.
- Article: Phosphatase Inhibitor Cocktail
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
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