200-100-6
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Scalability of Mobius®Single-Use Bioreactors
L 200 L 1000 L 2000 L
Model Prediction Emperical Data
90
91
92
93
94
95
96
97
98
99
100
0 2 4 6 8
MAGPIX®and Luminex 200™ - Multiplex Detection of Inflammation Pathway Analytes
mL)
IL-6
Luminex 200™ MAGPIX®
10
100
1,000
10,000
100,000
1 10 100 1,000 10,000 100,000
M
FI
Standard (pg/mL)
Luminex 200™ MAGPIX®
GM-CSF
CellASIC Cancer Brochure
250
200
200
150
100
100
50
0
0
y
ax
is
(µ
m
)
x axis (µm)
-50
-100
-100
-150
-200
-200
-250
-300
-300
10%
0%
Protocol: HUMAN PROINSULIN RIA KIT - 250 TUBES
,12 - 200 µL of 12.5 pM 100 µL 100 µL 100 µL 1.0 mL
13,14 - 200 µL of 25 pM 100 µL 100 µL 100 µL 1.0 mL
15,16 - 200 µL of 50
High Viscosity Tangential Flow Filtration (TFF) Applications
kD Ultracel Cassettes, QF = 6 L min-1 m-2
0
20
40
60
80
100
120
140
0
10
Process Guidance - Cellvento BHK-200
in Cellvento® BHK-200 medium +
10% DMSO, 1×107 viable cells/mL)
- Prepare a WCB (min. 100 tubes in CellventoTM BHK-200 medium +
10% DMSO, 1×107 viable cells/mL)
6
Process Guidance Cellvento™ BHK-200 Medium EMDM
• Subculture the cells at a seeding density of 6×105 viable cells/mL in Cellvento™ BHK-200 medium + 10% serum,
with a culture volume of 100 mL in a 250 mL shake bottle/Erlenmeyer in an incubator
Poster - Binding using SPR Biacore
0
RU
150
2e-6 4e-6 6e-6 1e-58e-6 12e-5
M
200
100
40
0
KD=12.3 µM
Concentration
R
e
sp
o
n
se
0
RU
20
2e-6 4e-6 6e-6
Evaluation of MultiScreen-MIC Plates in Chemotaxis Assays
1 3 4 5 6 8 9 10 11 12
A 10 800 800 800 800 200 200 200 200 200
B 10 800 800 800 800 200 200 200 200 200
C 10 800 800 800 800 200 200 200
Performance Evaluation and Cleanability Study using Pellicon®3 Cassettes with 30 kD Biomax®and Ultracel®Ultrafiltration Membranes
Ultracel® Cassettes, QF = 6 L min-1 m-2
0
20
40
60
80
100
120
140
0
10
Flyer Rediscover Thin Layer Chromatography from the world leader
20 x 10 200 µm 50 plates glass 1.05642.0001 5642-6
TLC Aluminum
oxide 60 F254
neutral
20 x 20 200 µm 25 sheets aluminum 1.05550.0001 5550-7
TLC PEI
cellulose F
20 x 20
Considerations for Dosing Accuracy in a New Carrier for Dry Powder Inhalation
300
200
100
0
To
ta
l E
ne
rg
y
[m
J]
0Test Number
Tip Speed [mm/s]
2
1 1
1 1
-100 -100 -100-100 -100 -100 -70-100 -100 -40 -10
4
Article: Virus filtration process development optimization
pH range (5.0–7.0). Performance increased
insignificantly to 100–200 L/m2 for all pH
levels and both area ratios (Figure 6). That
lack of effectiveness was probably
attributable to the higher
Mobius® and UltraMobius
Components
• 25 Mobius 200 Columns
• 25 Mobius 200 Filters
• Mobius Buffer Kit
1 ml RNase A Solution
100 ml Bacterial Resuspension Buffer (1)
100 ml Bacterial Lysis Buffer
Process Guidance Cellvento™ CHO-200 Medium
evaluation options
Feeding option 1
Culture day Addition order 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
Cellvento™ Feed-200 (% v/v) 1 3 6 6 6
Glucose 2 monitor daily and maintain at 4 g/L daily
Cys/Tyr stock solution
Milliflex-100 Single-Head Pump and Milliflex Sensor II Twin-Head Pump Sanitation User Guide
Milliflex-100 Single-Head Pump
and
Milliflex Sensor II Twin-Head Pump
Sanitation User Guide
MXP1 115 60, MXP1 230 50, MXPS 200 15, and MXPS 200 23
2 www.millipore.com Milliflex-100 Single-Head
Human Amyloid Beta and Tau Magnetic Bead Panel
Standard 6 200 µL 100 µL of Standard 7
Standard 5 200 µL 100 µL of Standard 6
Standard 4 200 µL 100 µL of Standard 5
Standard 3 200
Product Information Sheet - CHXACT
) and plate 100 to 200 µl per
well of a gelatin coated 96-well plate.
6. When cells are confluent (after 40 to 48 hr),
prepare the following samples by replacing the
medium with 200 µl growth
Aldrich Polymer Products Application - Ionx
42,866-3 50WX4-100 4 G 50-100 H 68 150 1.1 4.8 0-14
42,209-6 50WX4-200 4 G 100-200 H 68 150 1.1 4.8 0-14
42,867-1 50WX4-200R 4 G 100-200 H 68 150 1.1 4.8 0-14
21,748-4 50WX4-400 4 G 200-400 H 68 150 1.1
Data Sheet - 1.46429
Tubes 1.46271.0020 20 x 6 ml tubes
Tryptic Soy Agar 1.46429.0020 20 x 18 ml tubes
Tryptic Soy Agar 1.46429.0100 100 x 18 ml tubes
Tryptic Soy Agar 1.46457.0006 6 x 200 ml bottles
Data Sheet - 1.46457
Tubes 1.46271.0020 20 x 6 ml tubes
Tryptic Soy Agar 1.46429.0020 20 x 18 ml tubes
Tryptic Soy Agar 1.46429.0100 100 x 18 ml tubes
Tryptic Soy Agar 1.46457.0006 6 x 200 ml bottles
Data Sheet - 1.46375
Tubes 1.46271.0020 20 x 6 ml tubes
Tryptic Soy Agar 1.46429.0020 20 x 18 ml tubes
Tryptic Soy Agar 1.46429.0100 100 x 18 ml tubes
Tryptic Soy Agar 1.46457.0006 6 x 200 ml bottles
EdU Cell Proliferation Assay
cells twice with 200 µL of 3% BSA in PBS.
5. Proceed to Fluorescent EdU Detection steps.
6
Flow Cytometry
I. EdU
Protocol: Glucagon-Like Peptide-1 (Total) RIA Kit
,18 200 L 100 L of 500 pM 100 L 100 L 10 L 1.0 mL
19,20 200 L 100 L of 1000 pM 100 L 100 L 10 L 1.0 mL
21,22 200 L 100
Protocol- HND3MAG-39K
11.11
Standard 6 10 333.33 1,000 8.33 20 33.33
Standard 7 30 1,000 3,000 25 60 100
100 µL 100 µL 100 µL 100 µL 100 µL 100 µL
Mouse Metabolic Hormone Expanded Panel
Standard 6 200 µL 100 µL of Standard 7
Standard 5 200 µL 100 µL of Standard 6
Standard 4 200 µL 100 µL of Standard 5
Standard 3 200
Human IL-6 ELISA
Standard 7 200 µL
200 µL of reconstituted
standard
75
Standard 6 200 µL 200 µL of Standard 7 37.5
Standard 5 200 µL 200 µL of Standard 6 18.75
Standard 4
Protocol- HIGFBMAG-53K
Standard 6 8.33 83.33 16.67 166.67 333.33 33.33 16.67
Standard 7 25 250 50 500 1000 100 50
100 µL 100 µL 100 µL 100 µL 100 µL 100
Rat Thyroid Hormone Panel Kit
Standard 5 Standard 4 Standard 3 Standard 6 Standard2 Standard 1
100 µL 100 µL 100 µL 100 µL 100 µL
200 �L 200 �L
200 �L
200 �L
Mouse Immunoglobulin Isotyping Magnetic Bead Panel
Standard 6 200 µL 100 µL of Standard 7
Standard 5 200 µL 100 µL of Standard 6
Standard 4 200 µL 100 µL of Standard 5
Standard 3 200 µL 100 µL of
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