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Showing 1-30 of 721 results for "227-057-6" within Site Content
Ni Sepharose 6 Fast Flow for Protein Purification
Ni Sepharose 6 Fast Flow purifies histidine-tagged proteins efficiently, offering high cross-linked agarose beads with Ni2+ ions.
Oligonucleotide Standard 6 Mix LC-UV Analysis
Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]
To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.
Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
Dextran
Dextran polymer details: composed mainly of alpha-D-(1-6) linkages with varied branch lengths.
Performing a Separation or Removal of Albumin with HiTrap® Blue HP and Blue Sepharose 6 Fast Flow
This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.
Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Thermal Transitions of Homopolymers: Glass Transition & Melting Point
Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.
Sera Buy/Get Promotion - Terms and Conditions
Official terms and conditions for MilliporeSigma Sera Buy/Get promotion. Offer ends 12/6/24. Valid only in US and Canada.
Removal of Albumin Using Blue Sepharose® Chromatography Media
Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.
Enzymatic Assay of Alcohol Oxidase (EC 1.1.3.13)
To measure alcohol oxidase activity, this assay uses 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) and a continuous spectrophotometric rate determination at 405 nm.
Determination of Hydrocortisone from Topical Cream Using Discovery DSC-Si SPE and Reversed-Phase HPLC-UV
Using the method described in this report, an average absolute recovery and RSD value of 99.86 ± 6.99% (n=6) was observed, to determine an average of 1.02% hydrocortisone in topical cream.
Purification or Removal of DNA-Binding Proteins
This page shows how to purify or remove DNA-binding proteins with Heparin Sepharose High Performance, Heparin Sepharose 6 Fast Flow, Capto Heparin from Cytiva.
USDA FSIS STEC Guidance Implementation
Discover the expanded USDA FSIS verification testing for the 'Big 6' non-O157 STEC in beef products and explore accurate testing solutions and industry practices for enhanced food safety.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
Preservation of Moisture-Sensitive Chemical Reagents
Preserve reagent quality of air- and moisture-sensitive reagents using nitrogen or argon in crown-cap bottles with a 6 mm diameter hole in the crown-cap and a PTFE-faced rubber liner.
Determination of Water Content in Phenol Using Karl Fischer Titration
Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.
Performing a Separation of DNA binding proteins with Cytiva Products Based on Heparin
This page shows how to use heparin in the separation of DNA binding proteins used in HiTrap Heparin HP, HiPrep 16/10 Heparin FF and Heparin Sepharose 6 Fast Flow products from Cytiva.
Purification of NAD+ and ATP-dependent Kinases
Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.
Detect, Visualize and Quantify Single Post-Translational Modifications
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Formulation and Delivery US 2024
Join us at the Formulation and Delivery US conference at booth #6 to learn about our integrated offering for all process steps in pharmaceutical and biopharmaceutical manufacturing which includes products that meet the highest quality and purity standards with extensive
ChemMatrix® Resin for solid phase peptide synthesis
ChemMatrix® is a proprietary, 100% PEG (polyethylene glycol) based resin from PCAS BioMatrix.
Understanding and Successfully Applying Materials for Dye-Sensitized Solar Cells
While dye sensitization as the basis for color photography has been accepted for a very long time,1 attempts to use this principle for the conversion of solar light to electricity generally had resulted only in very low photocurrents, below 100
MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications
Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.
3D Cell Culture with Corning Microplates
Take a deep dive into the world of 3D cell culture courtesy of Corning and understand why the Corning® spheroid microplate can be an asset in the evolution of 3D cell culture.
Phyllosilicate Clay Minerals: Principles and Applications
We have discussed shortly the applications of 1:1 and 2:1 phyllosilicate material in membrane technology, as well as the basic geometric understanding of their layered structures.
Functional Biomaterials Synthesized by Double-Head Polymerization Agents
Over the past two decades, the rapid advance of controlled living polymerization (CLP) techniques.
Synthesis of Melting Gels Using Mono-Substituted and Di-Substituted Alkoxysiloxanes
Hybrid organic-inorganic sol-gel materials containing silica were first called “ORMOSILs” in 1984.
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