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SMC®Plate Washer Evaluation Kit
Maintenance
1 2 3 4 5 6 7 8 9 10 11 12 Row Mean SD CV
A 68 65 86 57 71 79 61 79 59 69 59 82 70 10 14
B 79 55 63
Innovative Detection of Listeria
positive result with cultivation based; § 64 LFGB method )
Number of Listeria
monocytogenes Positive Samples
food category n §64 LFGB HybriScan
meatproducts 72 42* (39) 39
fish 36 15 15
milk products
Instruction Manual: siIMPORTER™ siRNA and Plasmid DNA Transfection Reagent
in targeted protein levels
between 72 and 96 hours post transfection as a general
rule. Adjust times as necessary for the specific gene
target studied.
Cat. #64-101
siIMPORTER™ Transfection
The Effective Use of Protein Kinase Inhibitors
21 100 70 86 102 105 100 94
88 104 104 102 81 88 98 91 104 117 45 108 64 22 26 84 90 75 100 87 89 98
83 99 107 116 72 64 95 76
Data Sheet 1.13116.0500
Incubate the other set at
20-24 °C for 64-72 h.
Examine the plates as soon as they are removed from the incubators, if this is not
possible, store them at +2 °C to +8 °C and examine them within
The Effective Use of Protein Kinase Inhibitors
21 100 70 86 102 105 100 94
88 104 104 102 81 88 98 91 104 117 45 108 64 22 26 84 90 75 100 87 89 98
83 99 107 116 72 64 95 76
Dental Application Brochure
peroxide Thermal
initiator
94-36-0
2, 2'-(4-Methylphenylimino) diethanol Co-initiator 3077-12-1
N, N-Dimethyl-m-toluidine Co-initiator 121-72-2
2-(4-(Dimethylamino) phenyl) ethanol Co-initiator
Product Information Sheet - INHIB1
6 mg/mL (DMSO)
E-64 E3132 5 mg 2–8 C 10 M 357.4 20 mg/mL (water)
EDTA disodium salt ED2SS 50 g RT 1 mM 372.2 50 mg/mL (water)
Product Information Sheet - P1623
minute;
cycled 30 times using 92 °C for one minute, 57 °C for
one minute, 72 °C for two minutes. A final five-minute
extension at 72 °C was added at the end.
Results
Figure 1 shows amplification of
Application Note Anaerobic EM using ICR+
137 / 119 / 140
ø = 132 169/ 155 116 1.1 1.8 1.8
B. fragilis ATCC
25285
54 / 70 / 64
ø = 63 72/64 118 2.0 – 2.1 2.6 – 2.7 3.0 – 3.2
C.
Analysis of Antibiotic Sulfa Drugs Using Ascentis C18
Cramer
Acquisition System: Waters Alliance
2690 ID 9371
Notebook Reference: 1486-64
Peak IDs
1. Sulfanilamide
2. Sulfacetamide
3. Sulfadiazine
G002345
©2004 Sigma-Aldrich Co. T004087
Sulfathiazole
Product Information Sheet - C3240
Annealing
Extension
Plate Read (real-time)
94 °C
65 °C
72 °C
80 °C
15 sec
45 sec
1 min
1 sec
Incubate 72 °C
Data Sheet - L5535
For laboratory use only. Not for drug, household or
other uses.
Preparation Instructions
Suspend 64 grams of Liver Broth in 1000 ml of distilled
water. Soak for 15 minutes with occasional stirring.
Product Information Sheet - 92451
-life
• 2 years from date of shipping
Figure 1: Progress Isotherm
TS+ +25
NovAseptic®Mixer: Tank Plate, GMP ATEX Specification
M6 45º 64
(2.52)
— 4
(0.16)
1
(0.04)
1.8
(0.07)
GMP100 ATEX
AX5/33 54
(2.13)
90
(3.54)
68
(2.68)
50
(1.97)
56
(2.20)
— 19
(0.75)
56
(2.20)
NovAseptic®Mixer, Tank Plate, GMP and HS
(1.81) M6 45º
64
(2.52) —
4
(0.16)
1
(0.04)
1.8
(0.07) GMP100
GM5/33 54 (2.13)
90
(3.54)
68
(2.68)
50
(1.97) — —
19
(0.75)
56
(2.20) M6
Data Sheet - C9354
1996).
6. Williams, C., et al., Ann. N. Y. Acad. Sci., 889, 72
(1999).
7. Dubois, R. N., Aliment Pharmacol. Ther., 14, Suppl.
1, 64 (2000).
8. Langenbach, R., et al., Cell, 83, 483
DataSheet - S8830
which contains the
following inhibitor concentrations:
• AEBSF: 2 mM
• Phosphoramidon: 1 µM
• Bestatin: 130 µM
• E-64: 14 µM
• Leupeptin: 1 µM
59505 Galactosyltransferase Kit
71 71
dimethylsulfoxide 72 74 75
methanol 77 80 84
ethanol 80 85 86
acetone 76 76 67
dioxane 72 67 25
acetonitrile 69 66 55
MMP Activation Chart
2h
C
82
(70)
67
(64)
66.6*
F88QTFE; N-term & C-
term cleavage
1x
Okada, JBC267/'92/21712;
Shapiro JBC 270,1995,6351
MMP-10 Stromelysin-2 Same as
DataSheet - P8849
hydrochloride: aminopeptidases, such as
leucine aminopeptidase and alanyl
aminopeptidase2–5
• E-64 [N-(trans-Epoxysuccinyl)-L-leucine
4-guanidinobutylamide]: cysteine proteases, such
as calpain, papain
Evaluation of MultiScreen-MIC Plates in Invasion and Angiogenesis Assays
86 ± 8 72 ± 6
Plate 2 - - 57 ± 7 80 ± 11 65 ± 6 65 ± 9
Plate 3 - - 74 ± 13 62 ± 7 - -
3 plates per lot, with
Data Sheet - MSST0044
metalloproteinases 1 and rectal cancer patient
survival. a validation study. Eur. J. Cancer, 40, 64–
72 (2004).
4. Yoshikawa, T. et al., Intratumoral concentrations of
tissue inhibitor of matrix
Data Sheet - MSST0058
Cheng
Xue Za Zhi, 19(4), 697-702 ( 2002).
2. Bickel, M., The role of interleukin-8 in inflammation
and mechanisms of regulation. J. Periodontol.,
64(5 Suppl), 456-60 (1993).
3. Urquidi, V. et
Data Sheet - SAB4200165
, Biochem. Biophys.
Res. Commun., 354, 491-497 (2007).
5. Hadano, S., et al., FEBS Lett., 575, 64-70 (2004).
VS,ER,KAA,PHC,MAM 07/19-1
2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
Enhanced Sensitivity Immunogenicity (ADA) Assay - Next Generation SMC™ Technology
B + F
23.44 ng/mL
B + F
0 1 1 1 1
0.05 1 2 1 1
0.19 2 2 1 1
0.76 3 3 2 2
3.05 6 9 7 8
12.00 10 25 28 29
49 19 44 96
Data Sheet - H7909
agonist HU-210 on defensive-withdrawal
behavior in rats., J. Pharmacol. Exp. Ther. , 276,
56-64 (1996).
2. Berdyshev, E. V., et al., Cannabinoid-receptor-
independent cell signaling by
N-acylethanolamines
PureFlex™and PureFlex™ Plus White Paper
07-0 43
2-butanol 78-92-2 11
Butanal (TIC) 123-72-8 45
Pentanal (TIC) 110-62-3 105
Hexanal (TIC) 66-25-1 92
3,3,-dimethyl-2—butanone (TIC) 75-97-8 14
1N NaOH No
acetone 67-64-1 120
1-propene, 2-methyl
Data Sheet - SRP5017
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼64 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 63–72 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is
Data Sheet - SAB4200833
(2016).
2. Pilo, P., Front. Cell Infect. Microbiol., 8, 258
(2018).
3. McLendon, M.K. et al., Annu. Rev. Microbiol.,
60, 167-85 (2006).
4. Ancuta, P. et al., Infect. Immun., 64, 2041-6
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