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OmniPur Agarose Products
trademark of
EMD Chemicals Inc.
OmniPur® PCR Plus Agarose is a blend
of agarose developed to provide high
resolution of small nucleic acid
fragments and PCR products. It is
specifically designed for resolution
Application Note - RealTime PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
gel
electrophoresis (4% agarose) and visualized by ethidium bromide staining. Arrows indicate the size of the expected
PCR products. The bands in white boxes are non-specific PCR products and primer dimers
User Guide- DPLUS-50
User Guide
DirectLoad™ Plus
50 bp DNA Ladder
DPLUS-50 500 µL/vial
Storage
-20 °C for up to 24 months or 4 °C for up to
six months.
User Guide- DPLUS-1K
DirectLoad™ Plus
1 Kb DNA Ladder
DPLUS-1K 500 µL/vial
Storage
-20 °C for up to 24 months, 4 °C for up to 12 months,
or room temperature for
User Guide- DPLUS-100H
DirectLoad™ Plus
100 bp High DNA Ladder
DPLUS-100H 500 µL/vial
Storage
-20 °C for up to 24 months, 4 °C for up to 12 months,
or room temperature for
User Guide- DPLUS-100
DirectLoad™ Plus
100 bp DNA Ladder
DPLUS-100 500 µL/vial
Storage
-20 °C for up to 24 months, or 4 °C up to 12 months
or room temperature for up
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
standardization.
OmniPur® Agarose PCR Plus
For superior resolution of DNA fragments, especially small
fragments (PCR products), use OmniPur
Agarose PCR Plus. OmniPur Products represent
Product Information Sheet - APOPCR
or equivalent
• Agarose
• Ethidium bromide, 500 µg/ml, Product No. E1385
• PCR 100 bp low ladder, Product No. P1473
• Gel loading solutions, Product No. G2526 or
G7654
• PCR grade water, Product
Molecular Biology Reagents Insert: OmniPur®for Western Blotting
100 ml $30
Agarose 62-68°C melt range,
DNA Fragments >500kbp
2070 100 g $629
PCR plus Agarose 87-89°C melt range,
DNA Fragments >1000bp
2120 100 g $171
PCR plus Agarose 87-89°C melt range
Poster: Montage PCRµ96 and SEQ96: A Total Solution for Sequencing PCR-derived Templates
achieved using this platform and is suitable for the most
demanding applications.
1.5% agarose gel analysis of PCR products after
purification using Montage PCRµ96 or devices from
competitors Q & MN.
inNovations Newsletter #12
,500 U 71003-5 $950
NovaTaq PCR Kit 71005-3 $140
(includes complete set of qualified reagents for PCR)
NovaTaq PCR Kit PLUS 71006-3 $150
(includes PCR reagents plus Optimization Buffer for difficult
UltraClear Sequencing Reaction Clean-Up Kit
8
3
2
49
PCR PRODUCT PURIFICATION
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Recovery of 2 to 23 kb
DNA fragments purified
using GenElute Agarose
Spin Columns
Figure 2
Data Sheet - D7808
PCR Low Ladder Set (D7808) - Datasheet
PCR Low Ladder Marker Set
Product Code D 7808
Store at 2 to 8 °C
Product Description
The PCR Low Ladder Marker Set has been
Product Information Sheet - 4743814001
Check the PCR product on an agarose gel for size and specificity
using an appropriate size marker*.
• Purify the PCR product with the High Pure PCR Product Purification
Kit *.
The obtained PCR fragments
CpG WIZ®SOCS1 Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
CpG WIZ®VHL Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
CpGenome™ Direct Prep Bisulfite Modification Kit
Amplification Kit was used to amplify converted DNA
using methylation-specific PCR. Amplified products were
analyzed on a 2% agarose gel.
U: CpG WIZ ® Unmethylated Primer
M: CpG WIZ® Methylated Primer
inNovations Newsletter #18
This Issue!
BloodDirect™ PCR Buffer Kit, Human . . . . . . . . . . . . . . . . .19
BloodDirect PCR Buffer Kit, Mouse . . . . . . . . . .
CpG WIZ®RASSF1A Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
CpG WIZ®RB1 Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
CpG WIZ®GSTpi Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
CpG WIZ®Fragile X Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
Product Information Sheet - ID0010
After the digestion
remove a 3-5 µl aliquot of each reaction for agarose gel
analysis.
Step 3. Purification of PCR products
If PCR results reveal a single amplification band with a
reasonable yield
CpG WIZ® MGMT Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
CpG WIZ® DAP-kinase Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE, 2% agarose, 10% acrylamide, or
suitable high resolution agarose)
e. DNA markers (size
CpG WIZ®MGMT Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
Product Information Sheet - ID0030
After the digestion
remove a 3-5 µl aliquot of each reaction for agarose gel
analysis.
Step 3. Purification of PCR products
If PCR results reveal a single amplification band with a
reasonable yield
CpG WIZ®hMLH1 Amplification Kit
polymerase
c. "Hot start" PCR reagents (see Sec. II. Protocols).
d. Reagents for gel electrophoresis (1X TBE and 2% agarose, 10% acrylamide,
or suitable high resolution agarose)
e. DNA markers (size
Product Information Sheet - ID0040
After the digestion
remove a 3-5 µl aliquot of each reaction for agarose gel
analysis.
Step 3. Purification of PCR products
If PCR results reveal a single amplification band with a
reasonable yield
Product Information Sheet - 11811002001
Check the PCR product on an agarose gel for size and specificity
using an appropriate size marker*.
• Purify the PCR product with the High Pure PCR Product Purification
Kit *.
The obtained PCR fragments
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