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Overnight Express Autoinduction Systems
Protein Expression with Overnight Express™ Autoinduction Systems
Overnight Express™
Autoinduction Systems
Can EMD Chemicals make my protein expression process easier?
inNovations Newsletter #17
inNovations 17 June 2003
Protein expression
by autoinduction in E. coli
Higher yields and less work with the
Overnight Express™ Autoinduction System
page 3
Novagen Newsletter•Advanced products and
inNovations Newsletter #18
Express Autoinduction
System 2.
REFERENCES
1. Grabski, A., Mehler, M., and Drott, D.
(2003) inNovations 17, 3–6.
NEW PRODUCTS
Overnight Express™ Autoinduction System 2
Combines System 1 plus
Streamline Your Molecular Biology Workflow
Novagen Overnight Express™ Autoinduction
System
Simplify bacterial protein expression and increase
soluble protein yields using the The Overnight
Express™ Autoinduction System. No longer will
you have
Express yourself
Protocol Conventional Protocol
Overnight Express™ Autoinduction Systems
With Overnight Express™ Autoinduction Systems, a period of cell growth is
followed by spontaneous induction of protein
Radiance! Multisystem Protein Expression
Solution 15 ml 70527-3 $59
Overnight Express™
Autoinduction System 1
1 kit* 71300-3 $55
1 kit** 71300-4 $220
Overnight Express
Autoinduction System 2
1 kit* 71366-3 $98
1 kit** 71366-4 $392
TB184 Xa/LIC Cloning Kits
induced by growing cultures in Overnight Express™ Autoinduction Medium, or by adding IPTG to cultures in
conventional medium. Overnight Express Autoinduction Medium directs high-density cell growth in the
TB055 pET System Manual
suitable for growth of strains and expression of target proteins in the pET System. The
Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli without monitoring the culture
TB340 Duet Vectors
expression of the target ORF can be induced by using medium
prepared with Overnight Express™ Autoinduction System components (19), or by adding IPTG to a conventional
medium. Medium produced with Overnight
Effects of Initial Seeding Density and Fluid Perfusion Rate on Formation of Tissue-Engineered Bone
characterization. Biotechnol Bioeng 1999;64:580. [PubMed: 10404238]
21. Urist MR. Bone-formation by autoinduction. Science 1965;150:893. [PubMed: 5319761]
22. Urist MR, Delange RJ, Finerman GAM. Bone cell differentiation
Welcome To Bioprocessing
: G418,
Hygromycin, Kanamycin,
Ampicillin
• Cell culture media for
protein synthesis
autoinduction (without
IPTG) Overnight
Express™
LEARN MORE AT:
SigmaAldrich.com/molecularbiology
Protein Purification and Detection Tools
constructs were incubated at 30°C and target
protein expression was induced by Overnight Express™ Autoinduction System 1 (Cat. No. 71300). Following further
incubation for approximately 16 h at 30°C, the cultures