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BacMagic Systems pIEx/Bac Expression Vectors
BacMagic™ System: No Plaque Purification Method
Product Size Cat. No. Product Size Cat. No.
BacMagic™ DNA Kit 5 rxn 71545-3 BacMagic™ Transfection Kit 5 rxn 71546-3
BacMagic™-2 DNA Kit 5 rxn
Express yourself
efficiency transfection
BacMagic™ Systems‡
Ordering Information
Description Size Catalogue No.
BacMagic™-3 Transfection Kit‡ 5 reactions 72351-3
BacMagic™-3 DNA Kit‡ 5 reactions 72350-3
‡
TB163 Ek/LIC Cloning Kits
coli. Then isolate and cotransfect
plasmids with baculovirus DNA (BacVector®-1000, -2000, or -3000 Triple Cut Virus DNA or BacMagic™
DNA) into insect cells.
Vector
Fusion Tags Protease
Cleavage
Protein Purification and Detection Tools
baculovirus
• InsectDirect™ System
Plasmid-based protein expression
in 48 hours
• BacMagic™ DNA
Baculovirus expression
without plaque purification
New pIEx/Bac dual-purpose vectors
Product Information Sheet-11635379001
reaction. 1 vial,
0.5 ml
2 Rapid DNA Ligation Kit,
DNA Dilution Buffer, 5x conc.
To prepare DNA for ligation. 1 vial,
0.5 ml
3 Rapid DNA Ligation Kit,
T4 DNA Ligase
5 U/μl 1 vial
Product Information Sheet-11004760001
20 µl
2 Random Primed DNA
Labeling Kit,
dATP
0.5 mM
2′-deoxyadenosine-5′-
triphosphate in 10 mM Tris
buffer.
1 vial,
50 µl
3 Random Primed DNA
Labeling Kit,
dCTP
0.5 mM 2′-deoxycytidine
PureGenome™ Low Input NGS Library Construction Kit (EMD)
steps in 2 hours.
• No DNA purification step required.
• Proven to work in ChIP-Seq experiments.
• Kit includes 12 Illumina indexing barcodes and
Agencourt® AMPure® XP beads for DNA cleanup.
Product Information Sheet-11835246001
volume.
2 bags,
10 Filter tubes each
5 – Apoptotic DNA-
Ladder Kit,
Collection tubes
2 ml polypropylene tubes. 2 bags,
10 Collection tubes
each
6 red Apoptotic DNA-
Ladder Kit,
Positive
Product Information Sheet-11585614910
efficient random primed labeling of DNA.
1 vial,
50 μl
2 DIG-High Prime DNA Labeling
and Detection Starter Kit II,
DIG-labeled control DNA
• 5 μg/ml pBR328 DNA, linearized with Bam HI.
• Clear
User Guide - 905089
Action
Kit performance
deterioration
1. Check that the expiration date is not
exceeded.
2. Check that the product was stored at the
proper temperature.
Inappropriate
template DNA
1. Confirm
Product Information Sheet-11696505001
sigma-aldrich.com
2. How to Use this Product
y Version: 13 Agarose Gel DNA Extraction Kit
2. How to Use this Product
2.1. Protocols
Isolation of Electrophoretically Separated DNA Fragments with
High fidelity gene amplification
90%
← 150, 90%
1 2 3 4 5
Gel analysis and quantification of DNA
fragments isolated with the SpinPrep
GenElute High Performance(HP) Plasmid Kits: Plasmid DNA from a Midiprep orMaxiprep in 30 Minutes
digestion of plasmid
DNA purified using the
GenElute™ HP Maxiprep
Kit. Lane 1: 1 kb DNA
Ladder (Product Code D 0428);
Lane 2: 250 ng of undigested
plasmid DNA; Lane 3: EcoRI
and HindIII digestion of 250
DNA extraction kit for ChIC/CUT&RUN
ChIC/CUT&RUN kit manual. Mix briefly ensuring that the sample and binding buffer are
completely mixed.
2. For each sample, place a DNA Purification Column (Part # CS226333) into a 2 mL
Collection
User Manual - EC100
Spin
Tissue DNA 96 Kit
2
8
EC396-2EA
EC396-8EA
GenElute™-E Single Spin
Plant DNA 96 Kit
2
8
EC596-2EA
EC596-8EA
GenElute™-E Single Spin
Blood DNA 96 Kit
2
User Manual - EC400
Spin
Tissue DNA 96 Kit
2
8
EC396-2EA
EC396-8EA
GenElute™-E Single Spin
Plant DNA 96 Kit
2
8
EC596-2EA
EC596-8EA
GenElute™-E Single Spin
Blood DNA 96 Kit
2
User Guide - EC400
Spin
Tissue DNA 96 Kit
2
8
EC396-2EA
EC396-8EA
GenElute™-E Single Spin
Plant DNA 96 Kit
2
8
EC596-2EA
EC596-8EA
GenElute™-E Single Spin
Blood DNA 96 Kit
User Manual - EC500
Spin
Tissue DNA 96 Kit
2
8
EC396-2EA
EC396-8EA
GenElute™-E Single Spin
Plant DNA 96 Kit
2
8
EC596-2EA
EC596-8EA
GenElute™-E Single Spin
Blood DNA 96 Kit
2
Nano Juice™ Transfection Kit
Booster
= DNA
DNA
DNA DNADNA
DNADNADNA
DNA DNADNA
DNADNADNA
DNA DNA DNA
DNADNADNA DNADNADNA
1
3
2
3
CpG WIZ®VHL Amplification Kit
WIZ®
Amplification Kits, one microgram of purified DNA must undergo bisulfite
modification with the reagents contained in CpGenome™ DNA Modification
Kit (Cat. No. S7820).
STEP 2. Amplification
Product Information Sheet-11175025910
25°C, the kit is stable through the expiry date printed on the label.
Vial / Bottle Label Storage
1 pSPT18 DNA Store at −15 to −25°C.
2 pSPT19 DNA
3 Control DNA 1 pSPT18-Neo
4 Control DNA 2 pSPT19-Neo
User Guide - EC200
Spin
Tissue DNA 96 Kit
2
8
EC396-2EA
EC396-8EA
GenElute™-E Single Spin
Plant DNA 96 Kit
2
8
EC596-2EA
EC596-8EA
GenElute™-E Single Spin
Blood DNA 96 Kit
Whole Genome Amplification Product Listing
Chromosome 3 Chromosome 3
Figure 2. DNA from normal and tumorigenic human kidney cells were
amplified using the GenomePlex Single Cell WGA Kit. Amplified material
was hybridized to metaphase
CpG WIZ®SOCS1 Amplification Kit
WIZ®
Amplification Kits, one microgram of purified DNA must undergo bisulfite
modification with the reagents contained in CpGenome™ DNA Modification
Kit (Cat. No. S7820).
STEP 2. Amplification
"
Product Information Sheet - DNB600
concentration of
DNA purified from plasma or serum unless large
plasma/serum volumes have been processed.
1) 2100 Bioanalyzer DNA Quantification kits
DNA
1000
Kit
DNA
7500
Kit
DNA 12000
Kit
High
User Guide - EC300
Spin
Tissue DNA 96 Kit
2
8
EC396-2EA
EC396-8EA
GenElute™-E Single Spin
Plant DNA 96 Kit
2
8
EC596-2EA
EC596-8EA
GenElute™-E Single Spin
Blood DNA 96 Kit
Product Information Sheet-03143414001
isolation of plasmid DNA from bacterial cultures
Cat. No. 03 143 414 001 1 kit
20 preparations
Store the kit at +15 to +25°C.
Extract-N-Amp Seed PCR Kit: Rapid Genomic DNA Extraction from Seeds Coupled with PCR
es
10
molecular biology
Complete kit ensures fast extraction
and stable DNA
The Extract-N-Amp Seed PCR Kit was used to extract
and successfully amplify target DNA from various seed
sources including
CpG MethylQuest™ DNA Isolation Kit
Amplification Protocol
A total of 110 ng (2 ng/µl) of fragmented HeLa DNA is included in the kit, together with primers for 5
control samples plus 1 no-DNA control for each sample. Control PCR reactions
CpG WIZ® RARβ2 Amplification Kit (MilliporeSigma)
WIZ®
Amplification Kits, one microgram of purified DNA must undergo bisulfite
modification with the reagents contained in CpGenome™ DNA Modification
Kit (Cat. No. S7820).
STEP 2. Amplification
"
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