Bugbuster plus benzonase nuclease

History of Western Blotting

• ProteoExtract® kits for fractionation • CytoBuster™, BugBuster™ and CelLytic™ protein extraction reagents • Benzonase® nuclease • cOmplete™ and SIGMAFAST™ protease inhibitors • TruPAGE

Simplifying the Bacterial Recombinant Protein Workflow while Maximizing Recovery

With Benzonase® nuclease (not viscous, easy pipetting) A. B. C. Figure 3. BugBuster® reagent is superior to “Homebrew” lysis buffer and BugBuster® reagent with both Benzonase® nuclease and

Benzonase®Nuclease

in half. One half of the cells were lysed using BugBuster® Protein Extraction Reagent and the other half was lysed with BugBuster plus Benzonase® Nuclease following the recommend protocol. Following the

REFINE PROTEIN PREPARATION. Tools for better protein analysis

No. Bacteria BugBuster® Protein Extraction Reagent 70584 BugBuster® Master Mix 71456 BugBuster® Plus Benzonase® Nuclease 70750 BugBuster® Plus Lysonase™ Kit 71370 BugBuster® 10X Protein Extraction

Protein Preparation Workflow

No. Bacteria BugBuster® Protein Extraction Reagent 70584 BugBuster® Master Mix 71456 BugBuster® Plus Benzonase® Nuclease 70750 BugBuster® Plus Lysonase™ Kit 71370 BugBuster® 10X Protein Extraction

Protein Purification and Detection Tools

Lysis BugBuster® Protein Extraction Reagents . . . . . . . . . . . . . . . . . . . . . . . 8 BugBuster Plus Benzonase® Nuclease. . . . . . .

Ni-NTA HisﾕBind® Resins

units) Benzonase Nuclease per 1 ml BugBuster used for resuspension. b) Add 1KU rLysozyme Solution per 1 ml BugBuster (5 KU/g cell paste). c) Benzonase and rLysozyme can be pre-mixed with BugBuster for

TB334 rLysozyme Solution

units) Benzonase Nuclease per 1 ml lysis buffer used for resuspension. Benzonase is not recommended for nuclease free preparations. Protein purification may not remove Benzonase. Residual nuclease activity

Overnight Express Autoinduction Systems

Product Size Cat. No. Benzonase® Nuclease, Purity > 99% 10 KU 70664-3 Benzonase® Nuclease HC, Purity > 99% 25 KU 71206-3 BugBuster® Master Mix 100 ml 71456-3 500 ml 71456-4 BugBuster® Protein Extraction

inNovations Newsletter #18

stabi- lized recombinant lysozyme, and Benzonase® Nuclease, a recombinant nonspecific endonuclease that degrades all forms of DNA and RNA. The new BugBuster Plus Lysonase Kit combines the activities of

inNovations Newsletter #16

T7 Extracts Protein Extraction • rLysozyme™ Solution • Benzonase® Nuclease, Purity > 99% • Benzonase Nuclease, Purity > 90% • BugBuster® Protein Extraction Reagents • Lysonase™ Bioprocessing Reagent

inNovations Newsletter #15

post-induction and lysates produced by resuspending cell pellets in 1/10th volume BugBuster™ Protein Extraction Reagent + Benzonase® Nuclease + rLysozyme™ Solution according to the standard protocol. SDS-sample buffer

Simultaneous cell lysis and capture using the Amicon®Pro device

using BugBuster® Master Mix (Cat. No. 71456) according to the user manual. BugBuster® Master Mix combines BugBuster® extraction reagent with enzymatic agents, Benzonase® nuclease and

Evaluation of common protein extraction reagents in mammalian and bacteria lysates

homebrew method versus BugBuster® Protein Extraction Reagent, in the presence or absence of Benzonase® Nuclease and rLysozyme™ solutions. The results suggest that BugBuster® Extraction Reagent is more

inNovations Newsletter #14

rLysozyme™ Solution and Benzonase® Nuclease addition to the robotic protocol Figure 3 shows the processing enhance- ments resulting from the additions of rLysozyme and Benzonase Nuclease during the extraction

Protein Expression Infographic

A viscous protein sample is harder to pipette accurately. For DNA and RNA digestion: Benzonase® Nuclease (71206) P P P P TIP: Western or Immunoblotting = ~1-25 µg protein ELISA

TB340 Duet Vectors

and pre- mixed with Benzonase® Nuclease BugBuster Master Mix 71456-3 71456-4 100 ml 500 ml TB245 Use 5 ml/g wet cell paste. Tris-buffered and pre- mixed with Benzonase and rLysozyme™ Solution

Protein Extraction Infographic

A viscous protein sample is harder to pipette accurately. For DNA and RNA digestion: Benzonase® Nuclease (71206) P P P P TIP: Western or Immunoblotting = ~1-25 µg protein

inNovations Newsletter #17

Cat. No. BugBuster® Protein 100 ml 70584-3 Extraction Reagent 500 ml 70584-4 BugBuster HT Protein 100 ml 70922-3 Extraction Reagent 500 ml 70922-4 1 L 70922-5 Benzonase® Nuclease, 10 KU 70746

Automated purification of proteins from non-clarified E.coli lysate using BugBuster®Master Mix and PureProteome™ Nickel Magnetic Beads

well 1 LSKMAGS08 BugBuster® Master Mix 100 mL 71456-3 500 mL 71456-4 EMD Millipore, the M mark, PureProteome and rLysozyme are trademarks and Milli-Q, Benzonase and BugBuster are registered trademarks

The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

extracts. 24 Description Catalogue No. BugBuster 10X Protein Extraction Reagent 70921 BugBuster Plus Benzonase Nuclease 70750 YeastBuster Protein Extraction Reagent 71186

inNovations Newsletter #13

Novagen’s BugBuster™ Protein Extraction Reagent is a mixture of detergents capable of perforat- ing bacterial cells without denaturing pro- teins (5, 6). When used with Benzonase® Nuclease, non-viscous

New, combined lysis and purification reaction simplifies recombinant protein purification with magnetic beads

cells. This extraction reagent combines detergent-based lysis with the enzymatic agents, Benzonase® nuclease and rLysozyme™ enzyme, in a ready-to-use formulation. HARVEST EMD Millipore is a division

Amicon®Pro Affinity Concentration Kit - GST