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inNovations Newsletter #18
www.novagen.com
Product Size Cat. No.
BugBuster® 1 kit* 71370-3
Plus Lysonase™ Kit 1 kit† 71370-4
* includes 100 ml BugBuster Protein Extraction Reagent and
0.2 ml Lysonase Bioprocessing Reagent, sufficient
Protein Purification and Detection Tools
novagen
9Novagen • Protein Purification and Detection Tools
BugBuster® Plus Lysonase™ Kit
The BugBuster® Plus Lysonase™ Kit combines the activities of both
reagents to significantly increase protein
Benzonase®Nuclease
protein extracts. For efficient protein extraction with BugBuster Protein
Extraction Reagent, use 10 µl Lysonase per 1 g cell paste. In addition, Lysonase can be
used to enhance the effectiveness of non-detergent
inNovations Newsletter #16
µl B. subtilis BugBuster + Lysonase
2. 10 µl B. subtilis Tris + Lysonase
3. 10 µl E. faecalis BugBuster + Lysonase
4. 10 µl E. faecalis Tris + Lysonase
Use of BugBuster® and Lysonase™ Reagents
for
REFINE PROTEIN PREPARATION. Tools for better protein analysis
No.
Bacteria BugBuster® Protein Extraction Reagent 70584
BugBuster® Master Mix 71456
BugBuster® Plus Benzonase® Nuclease 70750
BugBuster® Plus Lysonase™ Kit 71370
BugBuster® 10X Protein Extraction
Protein Preparation Workflow
No.
Bacteria BugBuster® Protein Extraction Reagent 70584
BugBuster® Master Mix 71456
BugBuster® Plus Benzonase® Nuclease 70750
BugBuster® Plus Lysonase™ Kit 71370
BugBuster® 10X Protein Extraction
Overnight Express Autoinduction Systems
user-defined dilution and addition of buffer components. BugBuster 10X has all
of the bioprocessing benefits of standard BugBuster® Protein Extraction Reagent plus the freedom to control pH, reagent
concentration
TB334 rLysozyme Solution
brand of EMD Biosciences, Inc., an affiliate of Merck KGaA, Darmstadt, Germany. BugBuster, GST•Tag, His•Bind, His•Tag,
Lysonase, PopCulture, rLysozyme, RoboPop, Tuner, Veggie, the Novagen logo and Novagen
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression
Corporation.
BugBuster, Benzonase, Calbiochem, GeneJuice, NanoJuice , Novagen, His Tag, His Bind, and
Strep Tag are registered trademarks and YeastBuster, CytoBuster, rLysozyme, Lysonase, Nuc-
Buster
TB340 Duet Vectors
reserved. Benzonase®, BugBuster®, His•Bind®, PopCulture®, and
Novagen® are registered trademarks of Merck KGaA, Darmstadt, Germany. FRETworks™, GST•Tag™, His•Mag™, His•Tag™, LumiBlot™, Lysonase™,
Overnight
Ni-NTA HisユBind® Resins
....................32
*patent pending
BacVector, BugBuster, CytoBuster, FRETWorks, His•Bind, His•Tag, HSV•Tag, LumiBlot, Lysonase, PopCulture, rLysozyme, S•Tag, T7•Tag,
TriEx, YeastBuster
History of Western Blotting
• Recombinant
protein purification
affinity gels
• ProteoExtract® kits for fractionation
• CytoBuster™, BugBuster™ and CelLytic™ protein
extraction reagents
• Benzonase® nuclease
•
TB055 pET System Manual
BetaRed™ β-Galactosidase Assay Kit (Cat. No. 70978-3)
provides a rapid, sensitive measurement of β-galactosidase activity in cell extracts. Extracts prepared with BugBuster® Reagent,
PopCulture® Reagent
inNovations Newsletter #12
user-defined dilution and addition
of buffer components. BugBuster 10X has
all of the bioprocessing benefits of standard
BugBuster plus the freedom to control pH,
reagent concentration, and buffer additives
necessary
Simplifying the Bacterial Recombinant Protein Workflow while Maximizing Recovery
buffers that were used with
10 cycles of sonication.
Sonication-free cell lysis using BugBuster® reagent (plus
Benzonase® and rLysozyme™) was compared to three typical
lysis buffers (no Benzonase®/
inNovations Newsletter #15
Cell Pellet PAGE
E. coli BugBuster® Protein Extraction Reagent
BugBuster HT Protein Extraction Reagent
BugBuster (primary amine-free) Extraction Reagent
BugBuster 10X Protein Extraction Reagent
inNovations Newsletter #14
of
cells. BugBuster® Protein Extraction reagent
utilizes a mixture of detergents to disrupt
cell membranes and perforate bacterial cells
without denaturing soluble proteins (12,
13). BugBuster Reagent combined
inNovations Newsletter #17
Following induction, cells were harvested by centrifugation and extracted with BugBuster® HT Protein Extraction
Reagent plus rLysozyme™ Solution to produce a crude extract. Equal volumes (7 µl) of the extract
Brochure-Rethink Western Blotting
Preparation
Description Cat. No.
Lysis & Extraction Kits
BugBuster® Master Mix 71456
BugBuster® Plus Benzonase® Nuclease 70750
BugBuster® Protein Extraction Reagent (for bacterial lysis)
inNovations Newsletter #13
Plasmid)
His•Bind® Resin 10 ml 69670-3
50 ml 69670-4
100 ml 69670-5
His•Bind® Buffer Kit 69755-3
BugBuster™ Protein 100 ml 70584-3
Extraction Reagent 500 ml 70584-4
Benzonase® Nuclease,
Purity
Western Blotting Tools
www.emdmillipore.com/inhibitors
Description Catalogue No.
BugBuster® Protein Extraction Reagent (for bacterial lysis) 70584
BugBuster® Plus Benzonase® Nuclease
(nucleic acid degradation for more efficient
Express yourself
Catalogue No.
T7Select® 1-1 Cloning Kit 1 Kit 70010-3
T7Select® 415-1 Cloning Kit 1 Kit 70015-3
T7Select® 10-3 Cloning Kit 1 Kit 70550-3
T7Select® Packaging Kit 6 Extracts 70014-3
Vector Use
Display
Welcome To Bioprocessing
Preparation,
Purification and
Concentration
Extraction
• Lysis buffer for protein
extraction (Bugbuster® et
Cellytic®)
• Lysis enhancer
rLysozyme™, Nucleic acid
depletion : Benzonase®
• Proteases