Advanced gene editing

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Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Long Oligos

Techniques for long oligo synthesis and purification offer superior quality unmatched by other suppliers.

Small RNA and miRNA (microRNA) Sequencing

A review on reducing miRNA and other small RNAs sequencing bias with RealSeq®-AC RNA library preparation on the Illumina® RNA sequencing platform.

Small Molecule CRISPR Enhancers

Modulation of homology-directed repair (HDR) within the context of CRISPR-genome editing has led to the identification of small molecules that enhance CRISPR-mediated HDR efficiency in various cell types.

CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

CRISPR-based Gene Activation

Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.

MISSION® Lentiviral Controls

When conducting shRNA experiments, proper controls are a key element of experimental design. Proper use of controls permits accurate interpretation of knockdown results and provides assurance of the specificity of the observed phenotype. We offer a variety of positive, negative

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

shRNA Gene Families Sets

MISSION® shRNA Library gene sets mapped by Bioinformatics team for specific cellular pathways.

Validating CRISPR/Cas9-mediated Gene Editing

Validate CRISPR gene editing experiments easily with Sigma-Aldrich® T7E1 mismatch detection kit, ensuring successful editing.

Water for PCR Techniques

Learn how water impurities can affect PCR and related methodologies. High purity water purified using ultrafiltration is suitable for PCR and related techniques.

TRC Design and pLKO.1 Vector FAQs

MISSION® shRNA clones in pLKO.1-Puro vector allow bacterial culture propagation and mammalian cell selection for gene silencing studies.

Cas9-Mediated Recombineering for Kdo2-Lipid A

E. coli CRISPR/Cas-mediated Lambda-Red HR vector system enables gene editing via homology-directed repair.

MISSION^® shRNA Lentiviral Transduction

Lentiviruses can deliver significant amounts of genetic information into host cells. The resulting lentiviral particles were used to infect the human lung cancer cell line, H1299.

Nuclease-Free Water at Your Fingertips

An ultrafiltration cartridge can be placed at the outlet of a water purification system to deliver nuclease-free ultrapure water.

Water for Next-Generation Sequencing

Explore the essential role of water in next-generation sequencing for accurate and efficient genomic analysis

Water for pH Measurement and Buffer Preparation

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.