Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Prime Editing is a novel variation on CRISPR systems which expands the guide RNA’s responsibility to serve two purposes: (1) to guide Cas9 to a targeted genomic location, and (2) to serve as an RNA template to copy new sequences
Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.
The CRISPR/Cas genome editing system has revolutionized most every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA, or lentivirus.
Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic the human brain transcriptome - not to mention the blood-brain barrier - make genetic perturbation analysis
When conducting shRNA experiments, proper controls are a key element of experimental design. Proper use of controls permits accurate interpretation of knockdown results and provides assurance of the specificity of the observed phenotype. We offer a variety of positive, negative
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Targeted genome editing with Zinc finger nucleases (ZFNs) has a wide variety of applications, and three key modes of action have been a focus thus far: targeted gene knockouts, targeted gene integration, and targeted gene correction.
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
After you have performed a CRISPR experiment it is important to determine which gRNAs performed successfully editing. There are many ways to validate CRISPR gene editing experiments. A quick and easy way to check for cutting is by using the