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Precision Genetic Modifications
Our scientists’ years of experience and Sigma-Aldrich’s access to multiple advanced gene editing technologies allow us to offer a cell engineering service with unparalleled success. We can engineer a variety of modifications in your cell line of choice to address
Zinc Finger Nuclease Frequently Asked Questions
Zinc Finger Nuclease - frequently asked questions
How Do ZFNs Work?
CompoZr Zinc Finger Nucleases (ZFNs) are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections.
Methods for Enhancing Lentiviral Transduction Efficiency
An experiment to directly compare three methods of lentiviral transduction of Jurkat cells was conducted in order to determine the method that yields the greatest transduction efficiency.
Genome Engineering with CompoZr Custom Zinc Finger Nucleases (ZFNs)
Genome Editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Watch our video.
Safe Lentivirus Handling
Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.
Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process
The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).
Zinc Finger Nuclease References
Zinc Finger Nuclease References: Human, Rat, Mouse, Zebrafish, Plasmodium, Xenopus, Rainbow Trout, Mosquito, Pig, Rabbit, Cattle, CHO, ZFN Technology, Reviews, Posters, Label License
Prime Editing – An Exciting Combination of Natural and Engineered RNA-guided Genome Editing
Prime Editing is a novel variation on CRISPR systems which expands the guide RNA’s responsibility to serve two purposes: (1) to guide Cas9 to a targeted genomic location, and (2) to serve as an RNA template to copy new sequences
Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines
Small Molecule CRISPR Enhancers
The CRISPR-Cas9 system is an RNA-guided genome-editing tool that provides researchers a simple, easy, and quick way to modify the genomes of various organisms.
Long Oligos
Our experience with gene construction and microarray development provides us with insight into the potential difficulties of long oligo synthesis. We have developed techniques to purify long oligos, which are unmatched by other suppliers.
Small RNA and miRNA (microRNA) Sequencing
A review on reducing miRNA and other small RNAs sequencing bias with RealSeq®-AC RNA library preparation on the Illumina® RNA sequencing platform.
What Is ZFN Technology?
Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations.
Webinar: SygRNA®-Synthetic Two Part CRISPR RNA System
The CRISPR/Cas genome editing system has revolutionized most every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA, or lentivirus.
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
NitroGENIUS: Engineering E. coli to Fix Nitrogen and Regulating Transcription with Light
Endosymbiotic theory states that chloroplasts in plants originally came from a symbiotic relationship between prokaryotes.
CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments.
CRISPR on the Brain? Standardized Functional Genomics Tools Advance Neuroscience
Deciphering the genetics of neuroscience has always been challenging. Heterogeneous tissue microenvironments, complex genetic interactions and phenotypes, lack of model systems that accurately mimic the human brain transcriptome - not to mention the blood-brain barrier - make genetic perturbation analysis
Viral Invaders
Protection at the genetic level – a new line of defense against contamination in biopharmaceutical manufacturing.
CRISPR/CAS9 Gene Editing Protocol for Human Induced Pluripotent Stem Cells (iPSCs)
This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs).
MISSION® Lentiviral Controls
When conducting shRNA experiments, proper controls are a key element of experimental design. Proper use of controls permits accurate interpretation of knockdown results and provides assurance of the specificity of the observed phenotype. We offer a variety of positive, negative
CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein
Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.
MVM Resistance Through Genetic Engineering
Contamination in the manufacturing process can be a rare but catastrophic event costing a company approximately $5-7 million per contamination.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Targeted Genome Editing Using Engineered Zinc Finger Nucleases
Targeted genome editing with Zinc finger nucleases (ZFNs) has a wide variety of applications, and three key modes of action have been a focus thus far: targeted gene knockouts, targeted gene integration, and targeted gene correction.
CRISPR/Cas-GFP Vectors for Rapid Expression Verification and Enrichment of Genome Edited Cells
View experimental data showing crispr/cas expression and enrichment using FACS
Bacterial Transformation
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
Validating CRISPR/Cas9-mediated Gene Editing
After you have performed a CRISPR experiment it is important to determine which gRNAs performed successfully editing. There are many ways to validate CRISPR gene editing experiments. A quick and easy way to check for cutting is by using the
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
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