Cloning and Expression

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Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Restriction Enzyme Cloning Glossary

Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)

A helpful chart for selection of correct nucleic acid modifying enzyme.

Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Transforming E.coli with Engineered Plasmid

Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.

Cloning & Expression Vectors

Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Blue-White Screening & Protocols for Colony Selection

Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.

Competent Cell Selection & E. coli Markers Guide

Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.

FLAG® Tag Bacterial and Mammalian Expression Vectors

FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.

3xFLAG® System Expression Vectors for Ultra-Sens

The 3xFLAG system enhances fusion protein detection, offering improved sensitivity over traditional methods.

Synthesis of Polyamino Acids

Polyamino acids adopt ordered conformations with unique biological properties and functions like α-helices and β-sheets.

Plasmid Product Nomenclature

SnapFast system offers versatile plasmid cloning with pre-designed DNA sections for easy incorporation or transfer between plasmids.

Genotypes, Phenotypes and Markers

A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

T7 Promoter System

Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

Cloning Genes-of-Interest into a Plasmid Vector

This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.

Simplicon™ in vivo Protein Expression System

Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

Cloning Troubleshooting

Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..