MilliporeSigma
Search Within
Applications
Content Type

DNA and RNA purification

Applied Filters:
facet applications:DNA and RNA purification
facet content type:Protocol
Rapid Genotyping of Mouse Tissue Using Extract-N-Amp Tissue PCR Kit
Genomic detection of DNA via PCR amplification and detection on an electrophoretic gel is a standard way that the genotype of a tissue sample is determined. Conventional preparation of tissues for PCR-ready DNA often take several hours to days, depending
Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit
The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
REDExtract-N-Amp™ Tissue PCR Kit Protocol
The exrract-N-Amp kit protocol provides a rapid DNA extraction method that leads to a PCR-ready sample in 15 minutes. Optimized PCR ReadyMix yields successful and clean amplification. RED loading dye allows for sample tracking.
Protector RNase Inhibitor Protocol & Troubleshooting
The addition of Protector RNase Inhibitor to cell pellets is not recommended. Protector RNase Inhibitor as a 50kDa protein will most probably not permeate into intact cells. If the cell pellet is immediately frozen at -80 °C, this will be
SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol
The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
DNase I Recombinant, RNase-free Protocol
DNase I from bovine pancreas is a glycoprotein of Mr 37000. A special procedure is used to remove RNases from the DNase preparation.
Extract-N-Amp™ Tissue PCR Protocol for Formalin-fixed Paraffin Embedded Sections
The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.
Reliable DNA extraction from Whatman™ FTA cards
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
RNA Stabilization at Room Temperature in RNAstable®
Quantitative RT-PCR analysis demonstrates successful amplification of RNA templates that were stored dry in RNAstable® for 29 months at room temperature and under accelerated aging conditions equivalent to 12 years of room temperature storage.
RNase, DNase-free Protocol
0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.
Deoxyribonuclease I
Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Biological Sample Collection, Transport, Archiving, and Purification for Nucleic Acid Analysis
A number of options are available for sample collection, transport, archiving, and DNA purification.
Genomic DNA Purification from Sample Applied to FTA Cards
This page shows genomic DNA purification from sample applied to FTA cards from Cytiva.
Saliva DNA Extraction & WGA Amplification Protocol
Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.
Genomic DNA Purification using illustra™ GenomicPrep Mini Spin Kits
This page shows Genomic DNA purification using illustra™ genomicPrep Mini Spin Kits from Cytiva.
DNAgard® Blood Protocol
DNAgard Blood is designed for shipping and storage of DNA in mammalian whole blood. DNA in blood is protected by the unique stabilization properties of DNAgard Blood
Plant-Based PCR Inhibitors During DNA Extraction and PCR Master Mix Performance
Evaluating the effects of PCR inhibitors and PCR enzyme selection using plant genomic DNA, GenElute™-E Single Spin Plant Extraction Kit, KOD-One™ PCR Master Mix and competitor high fidelity PCR master mixes.
GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
REDExtract-N-Amp™ Plant PCR Kit Protocol
The REDExtract-N-Amp Plant PCR kit includes an inert loading dye in the ReadyMix for easy gel loading after amplification. This protocol provides a rapid protocol to extract plant genomic DNA for amplification using the optimized ReadyMix.
Instructions for EasiCollect™
This document shows intructions for EasiCollect™ device from Cytiva.
Extract-N-Amp™ FFPE Tissue PCR Protocol
Protocol for PCR amplification of formalin-fixed paraffin embedded animal tissues using the Extract-N-Amp Tissue PCR kit.
Extract-N-Amp™ Tissue PCR Kit Protocol
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
GenElute™ HP Plasmid Maxiprep Procedure
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
Extract-N-Amp™ Plant PCR Kits Protocol
The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.
Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits
Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.
Plant/Fungi DNA Isolation Kit Protocol
The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.
GenElute™ PCR Clean-Up Procedure
Follow this procedure to rapidly purify single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from excess primers, nucleotides, DNA polymerase, oil and salts using the GenElute™ PCR Clean-up Kit.
Plant/Fungi Total RNA Purification Kit
The Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from a wide range of plant and filamentous fungal species.
Page 1 of 2