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Histone Modification and Chromatin Remodeling | Epigenetics
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
Extract-N-Amp™ Tissue Feature Article
Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
Long-term Storage of Blood in DNAgard® Blood
Two separate studies were conducted to analyze long-term storage of blood in DNAgard Blood stabilization reagent.
Extract-N-Amp™ Blood PCR Kit Protocol
The Extract-N-Amp Blood PCR Kits contain all the reagents needed to rapidly extract and amplify human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.
Genomic DNA Preparation Troubleshooting
Description of common problems that can occur during genomic DNA preparation
Procedure for Purifying and Concentrating DNA using illustra™ GFX 96 PCR Purification Kit
This page shows the procedure for purifying and concentrating DNA using illustra™ GFX 96 PCR Purification Kit from Cytiva.
Plasmid DNA Preparation Troubleshooting
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
RNA Preparation Troubleshooting
This page shows possible causes and solutions for problems that may occur during RNA preparation.
DNA and RNA Quantification
An overview of methods for quantifying DNA and RNA, how to measure DNA and RNA concentration and yield, and how to assess purity of nucleic acid samples.
Sera-Mag™ and Sera-Mag SpeedBeads Magnetic Particles
Sera-Mag and Sera-Mag SpeedBeads provide cost effective magnetic bead separation technology for molecular biology applications, nucleic acid isolation, and research immunoassays.
Biomatrica® offers an innovative alternative to reliance on cold chain logistics
Biomatrica® products enable researchers to stabilize, ship, and store biological samples for long periods of time.
Protein Affinity Chromatography
Affinity chromatography is the process of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. This process allows for the highly specific and efficient purification of many diverse proteins and other compounds. The process requires
Sodium acetate buffer solution for molecular biology
Sodium Acetate Buffer provider. 5+ decades providing high-quality buffer products that maintain consistent performance for molecular biology .
Oligonucleotide Purification
This technical bulletin will help you select the best purification option for your oligo, depending upon the oligo type and its intended application.
High Pure Technology and Silica Adsorption Kits
The High Pure and silica adsorption kits developed by Roche depend on the tendency of nucleic acids to adsorb to silica (glass) in the presence of a chaotropic salt such as sodium iodide (NaI), guanidine thiocyanate or guanidine hydrochloride [Melzak
Stabilizing RNA at room temperature in RNAstable®
RNAstable® is a novel preservation product developed to protect RNA from degradation during storage or shipment at ambient temperatures. The synthetic storage medium is based on the natural principles of anhydrobiosis (meaning “life without water”), a biological mechanism employed by some organisms
ECACC Nucleic Acids
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
Ambient Temperature Stabilization of Genomic DNA in Saliva Samples
Saliva provides an alternative to blood as a biological fluid in diagnostic applications.
Extract-N-Amp™ Tissue Feature Article
The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the
Extract-N-Amp™ Plant Tissue PCR Kits
The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that
Genomic DNA Sample Purification and Quality Assessment
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of
KAPA Express Extract Kit FAQs
Frequently asked questions (FAQs) for KAPA Express Extract Kit.
RNA Isolation from Blood, Cultured Cells, and Animal or Plant Tissue on Whatman™ FTA™ Cards
This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Downstream Applications after Genomic DNA Purification
This page shows downstream applications after genomic DNA purification.
Downstream Applications for Plasmid DNA and Substrates
This page shows downstream applications for plasmid DNA and substances that may interfere in each.
General Considerations for Plasmid DNA Preparation
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method
In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome
Lentiviral Packaging
The goal of this exercise is to learn lentiviral packaging. For this example we will be using the HEK 293T cell line.
Impact of GenElute™-E Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes
Impact of Purification Method on Accuracy of DNA Quantitation and Downstream Enzymatic Processes. Evaluation of the purity of genomic DNA by UV spectrophotometry, gel electrophoresis, and downstream qPCR using GenElute™-E DNA purification kits.
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