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Lentiviral Production Using X-tremeGENE HP Transfection Reagent
Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.
Lentiviral Transduction Protocol
Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.
CRISPR Cas 9 Nuclease RNA-guided Genome Editing
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Antibiotic Optimization for Cell Transduction Using a Cytotoxicity Profile
Experimentally determine the appropriate antibiotic concentration for selecting stable cell lines. This titration protocol can determine the lowest concentration of puromycin needed to efficiently select transduced cells.
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors
Protocol for Transduction of Human Embryonic Stem Cells (hESCs) using Lentiviral Vectors