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A validated amino acid analysis assay for accurate quantification of stable isotope labeled protein reference materials: SIL thyroglobulin case study
With the emergence of mass spectrometry for the clinical measurement of proteins in biological matrices, the development of biological reference materials will continue to grow in importance.
UHPLC Analysis of Conjugated Estrogens on Ascentis ® Express C18
Separation of Estriol 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) 17-sulfate dipotassium salt; Estriol 3-sulfate sodium salt; β-Estradiol 3,17-disulfate dipotassium salt, ≥95%; β-Estradiol 17-(β-D-glucuronide) sodium salt; β-Estradiol 3-(β-D-glucuronide) sodium salt; Estrone 3-(β-D-glucuronide) sodium salt; β-Estradiol 3-sulfate sodium salt, ≥93%; Estriol, ≥97%; Estrone...
Ascentis ® Express HILIC U/HPLC Columns
For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.
HPLC Analysis of Sibutramine Enantiomers on Astec® CHIROBIOTIC® V
Separation of Sibutramine hydrochloride monohydrate, ≥98% (HPLC), solid
LC/MS/MS Analysis of Testosterone and Metabolites on Ascentis® Express C18
Separation of Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material; 17α-Methyltestosterone solution, 1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material; 5α-Dihydrotestosterone (DHT) solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified...
LC/MS Analysis of Ethanol Metabolites in Urine on Ascentis® Express OH5 after Dilution with Deuterated Internal Standards
The method shown uses HILIC mode on an Ascentis® Express OH5 column to retain both analytes well, making it very likely to be more robust and reliable, as well as highly MS-friendly.
HPLC Analysis of Cisplatin and Monohydrated Cisplatin on SeQuant ZIC-HILIC
Antineoplastic agents separated with excellent resolution.
LC/MS Analysis of Antiarrhythmic Drugs and Metabolites on Ascentis® Express HILIC
The benefit of HILIC over traditional reversed-phase chromatography is two-fold for both sample introduction and analyte detection. First, the high acetonitrile concentration of HILIC mobile phases allows for direct analysis of precipitated plasma samples without the need for additional sample...
HPLC Analysis of Xanthines on Purospher® STAR RP-18 endcapped
Xanthine is a purine base found in most human body tissues and fluids as well as in other organisms. Methylated xanthines (methylxanthines), which include caffeine, paraxanthine, theobromine, and theophylline, commonly used for their effects as mild stiµlants and as bronchodilators...
HPLC Analysis of Z-Drugs on Ascentis® Express ES-Cyano 2.0 μm
An rapid method for the siµLtaneous determination of the Z-drugs or sleep aids: zopiclone, zolpidem, and zaleplon is presented here. The need for greater analytical capacity and throughput for the analysis of sleep aid medicines (Z-drugs) in forensic toxicology laboratories...
Ascentis® Express RP-Amide Columns for HPLC and UHPLC
Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.
HPLC Analysis of Proteins on Proteomix® SCX-NP5
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;...
HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
Chromatography Profile Analysis of Phosphorothioate Oligonucleotides
The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular...
HPLC Analysis of Methylphenobarbital (Mephobarbital) Enantiomers on Astec® CHIROBIOTIC® V
Separation of Mephobarbital, United States Pharmacopeia (USP) Reference Standard
UHPLC/MS Analysis of Diazepam and Metabolites on Titan™ C18, Comparison of Flow Rate and Column Length
Separation of Oxazepam; Desmethyldiazepam, analytical standard; Temazepam, analytical standard; Diazepam
Ascentis® Express OH5 U/HPLC Columns
High-speed Ascentis® Express OH5 HPLC columns are designed for enhanced HILIC partitioning and limited ion exchange retention
How to Extend Liquid Chromatography Column Life: Regenerate or Use Guard Columns
What is causing aging of a column? Is the regeneration/washing of the column a reasonable approach to extend the lifetime of a column? What are the risks & challenges? Learn more in this article.
Determination of Sucralose in Soy Sauce using HPLC-ELSD following the Chinese National Standard (GB) Method
The most frequently used synthetic sweeteners are: saccharin, cyclamate, aspartame, and sucralose (E955), and this application illustrate the analysis of sucralose from soy sauce following the current Chinese national standard method.
Ascentis® Express RP-Amide HPLC Columns
Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.
HPLC Analysis of Proteins on TSKgel® BioAssist S
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;...
Peptide Sample Amount Determination
Determining the amount of desired product in a peptide sample.
Oligonucleotide Quality Control by Analytical HPLC
In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.
HPLC Analysis of Sugars by HILIC Chromatography using Ascentis® Express OH-5 Column with ELSD Detection
Separation of Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material; Sucrose; Maltitol, Pharmaceutical Secondary Standard; Certified Reference Material
Application Note – Peroxidase in Vegetables
Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.
HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan...
HPLC Analysis of Sugars on Ascentis® Si
Separation of Sucrose; Maltose; Maltotriose
HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size
Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis;...
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