Mammalian cell culture

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Protocol for creating create stable cell lines from both dividing and non-dividing cells for use with a lentiviral delivery system.

Identification of Cell Culture Media with Raman Spectroscopy

Identification of cell culture media samples by traditional LC methods, such as amino acid or vitamin analysis, has high costs, and requires significant analytical expertise and laboratory space.

Human Hematopoietic Progenitor Cells (HPC) and Growth Media

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages.

Hybridoma Fusion Partners Cell Lines

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Palmitic Acid in Cell Culture

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Alternative High-Yield Cell Culture Systems

Solutions for high-yield cell culture scale-up applications including multi-layered flasks (HyperFlasks/CellStacks), roller bottles, spinner and shaker flasks. Free ECACC handbook download.

Cultivating Consistency: Tips and Tricks for Successful Cell Cultures

Properly optimizing workflows and utilizing the latest best practices are the keys to obtaining consistent results and advancing research.

Cell Growth to Confluence

Monitor cell culture daily to assess the morphology and density of the cell. Watch video to know the concepts of cell growth and confluence.

Williams' Medium E Formulation

Cell Analysis Best Practices

How do you assess the state of your cell culture? We’ve got some best practices to share.

Lipoic (Thioctic) Acid, in Cell Culture

How lipoic acid (thioctic acid) and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

RPMI-1640 Media

RPMI-1640 is used for the culture of human normal and neoplastic leukocytes, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes.

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Citrate Serum-Free Medium Supplement

Citrate is one of many cell culture components that affects the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins such as monoclonal antibodies.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Cell Culture Protocol 10: Testing Cells for Mycoplasma Contamination by Hoechst DNA Staining

Cell culture protocol for testing cell lines for mycoplasma contamination using indirect DNA staining using Hoechst 33342.

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

Lipid-induced Insulin Resistance

Lipid Induced Insulin Resistance

VEGF Pathway

Read review on VEGF signaling pathway (Vascular endothelial growth factor (VEGF)/VEGF receptor) and find related products.

Dulbecco's Modified Eagle's Medium (DMEM) Formulation

Heparin Versus Heparin Sulfate Proteoglycans

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for

Cell Line Cross-Contamination and Misidentification; Common Cell Culture Problems

Cell line cross-contamination and misidentification can be solved with authentication and conscientious sourcing.

Cell Culture Contamination Troubleshooting

Common Cell Culture Problems: Contamination is easily the most common problem encountered in cell culture laboratories, sometimes with very serious consequence.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Ferric and Ferrous Iron in Cell Culture

Importance and uses of ferric and ferrous iron in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

T Cell Expansion and Viability Using Stericup^®-filtered Media

Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

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