Protein concentration and buffer exchange

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Urine Concentration with Amicon® Ultra Centrifugal Filters

Accurate measurement of specific proteins in urine is important for diagnosing and managing diseases.

Rapid Antibody Concentration with Amicon® Ultra Centrifugal Filters

Purification protocols for immunoglobulins generally include affinity binding to Protein A or G chromatography media, washing unbound proteins, and eluting with a low pH buffer.

A Simple Strategy for Protein Enrichment Using Ultrafiltration

The human plasma proteome is a vast resource for biomarker discovery and investigation.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

Large volume concentration and diafiltration with Amicon® Stirred Cells

Demonstrating the effective use of Amicon Stirred Cell for large volume concentration through diafiltration.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters

Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Detergent Removal with Amicon® Pro Centrifugal Filters

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

Amicon® Stirred Cells

User guide for Amicon® Stirred cells including features, operation, specifications, and troubleshooting.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Rapid, Ultrafiltration-based Antibody Purification

Ultrafiltration-based MAb purification offers faster, higher recovery compared to hybridoma screening.

Buffer Reference Center

Choose and prepare a biological buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature.

Simple dialysis of Small Volume Samples using V Series Membranes

This article is a protocol for Simple dialysis of small volumes using V Series membranes

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Small-scale Antibody Purification With Buffer Exchange and Concentration: Amicon® Pro System

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

Desalting Samples with Mini Dialysis Kit

Mini Dialysis Kit from Cytiva minimizes sample handling and loss, reducing streaking on 2-D gels from contaminants.

Extracellular Vesicle Protocol

Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.